Xing Guo-Sheng, Lou Jian-Shi, Wang Zhi-Bin, Yu Shun-Lu, Wang Yi, Sheng Li
The Research Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China.
Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(18):1909-13.
To investigate the effects of the decoction of Rhizoma Dioscorea septemlobae (RD) on the bone metabolism in ovariectomized rats.
Thirty female, 3-month-old Wistar rats without pregnancy and deliver were randomly divided into 6 groups: sham (sham-operation), ovariectomy (OVX), OVX + diethylstilbestrol, OVX + high dose RD (4 g x kg(-1) x d(-1)), OVX + middle dose RD (2 g x kg(-1) x d(-1)) and OVX + low dose RD (1 g x kg(-1) x d(-1)) (n = 5 in every group). After 12-week period of continuous treatment, the urinary samples and blood samples were collected for the determination of serum estrodiol (E2), calcium (Ca), phosphorus (P), bone glaprotein (BGP), alkaline phosphatase (ALP), urinary calcium/creatinine (Ca/Cr), phosphorus/ creatinine (P/Cr) and deoxypyridioline/creatinine (DPD/Cr). The uteri were removed and weighed. The bone mineral density (BMD) and the biomechanical parameters of the femur of the rats in every group were determined, respectively.
The coefficient of uteri in every dose group of OVX + RD was significantly higher than that in the OVX group (P < 0.01). The concentration of serum ALP, BGP and urinary DPD/Cr, Ca/Cr in the OVX group was significantly higher than that in the sham group (P < 0.05), respectively, However, that in the every dose of OVX + RD was lower than that in the OVX group, respectively. There was no significan difference in the concentration of serum Ca, P and urinary P/Cr in every group, respectively. The bone mineral density (BMD) in the OVX group was (0.032 +/- 0.007) g x cm(-2) and was significantly lower than that in the sham group (P < 0.01). However, the value in the group of every dose OVX + RD was significantly higher than that in the OVX group (P < 0.05, P < 0.01), respectively. The maximum loading, deflection and the maximum strain of the femur in the OVX group were (125.78 +/- 15.48) N, (1.87 +/- 0.22) mm, (9.34 +/- 1.10) % and were significantly lower than those in the sham group (P < 0.05, P < 0.01), respectively. The maximum loading and maximum stress were increased in different extent in the every dose group of OVX + RD, respectively.
The decoction of RD can inhibit bone absorption, decline bone turnover and improve the loss of bone in ovariectomized rats.
探讨绵萆薢水煎剂对去卵巢大鼠骨代谢的影响。
选取30只3月龄未孕未产雌性Wistar大鼠，随机分为6组：假手术组（假手术）、去卵巢组（OVX）、去卵巢+己烯雌酚组、去卵巢+高剂量绵萆薢组（4 g·kg⁻¹·d⁻¹）、去卵巢+中剂量绵萆薢组（2 g·kg⁻¹·d⁻¹）和去卵巢+低剂量绵萆薢组（1 g·kg⁻¹·d⁻¹）（每组n = 5）。连续治疗12周后，采集尿液和血液样本，测定血清雌二醇（E2）、钙（Ca）、磷（P）、骨钙素（BGP）、碱性磷酸酶（ALP）、尿钙/肌酐（Ca/Cr）、磷/肌酐（P/Cr）和脱氧吡啶啉/肌酐（DPD/Cr）。取出子宫称重。分别测定各组大鼠股骨的骨密度（BMD）和生物力学参数。
OVX +绵萆薢各剂量组子宫系数均显著高于OVX组（P < 0.01）。OVX组血清ALP、BGP浓度及尿DPD/Cr、Ca/Cr均显著高于假手术组（P < 0.05），而OVX +绵萆薢各剂量组上述指标均低于OVX组。各组血清Ca、P浓度及尿P/Cr差异均无统计学意义。OVX组骨密度为（0.032±0.007）g·cm⁻²，显著低于假手术组（P < 0.01），而OVX +绵萆薢各剂量组骨密度均显著高于OVX组（分别为P < 0.05，P < 0.01）。OVX组股骨最大载荷、挠度和最大应变分别为（125.78±15.48）N、（1.87±0.22）mm、（9.34±1.10）%，均显著低于假手术组（分别为P < 0.05，P < 0.01）。OVX +绵萆薢各剂量组最大载荷和最大应力均有不同程度增加。
绵萆薢水煎剂可抑制去卵巢大鼠骨吸收，降低骨转换，改善骨丢失。
