Hudolin Tvrtko, Kastelan Zeljko, Derezić Danijel, Basić-Jukić Nikolina, Cesare Spagnoli Giulio, Juretić Antonio, Jezek Davor
University Department of Urology, Zagreb University Hospital Center, Kispatićeva 12, HR-10000 Zagreb, Croatia.
Acta Dermatovenerol Croat. 2009;17(2):103-7.
Immunohistochemical expression of MAGE-A1, MAGE-A3/4 and NY-ESO-1/LAGE-1 cancer testis antigens (CTA) was assessed in 24 fetal testes from 15th to 36th week of gestation. Three monoclonal antibodies were used for immunohistochemical staining: 77B recognizing MAGE-A1, 57B recognizing multiple MAGE-A CTA, and D8.38 recognizing NY-ESO-1/LAGE-1. Expression of MAGE-A1 was not observed in fetal testis samples, whereas multi-MAGE-A and NY-ESO-1/LAGE-1 specific reagents stained pro-spermatogonia in all samples with different expression levels during the period of fetal development observed. Significant expression of MAGE-A3/4 and almost continuous expression of NY-ESO-1 in fetal testes after 22nd week of gestation suggested their important role in the development of sex cords and pro-spermatogonia in particular.
对妊娠15至36周的24个胎儿睾丸进行了黑色素瘤抗原基因A1（MAGE-A1）、MAGE-A3/4和纽约食管鳞状细胞癌-1/淋巴细胞抗原6基因家族成员A1（NY-ESO-1/LAGE-1）等癌胚抗原（CTA）的免疫组化表达评估。使用三种单克隆抗体进行免疫组化染色：识别MAGE-A1的77B、识别多种MAGE-A CTA的57B以及识别NY-ESO-1/LAGE-1的D8.38。在胎儿睾丸样本中未观察到MAGE-A1的表达，而多MAGE-A和NY-ESO-1/LAGE-1特异性试剂在观察到的胎儿发育期间的所有样本中均对精原细胞进行了染色，且表达水平不同。妊娠22周后胎儿睾丸中MAGE-A3/4的显著表达以及NY-ESO-1几乎持续的表达表明它们在性索尤其是精原细胞的发育中起重要作用。
