Akram Zertashia, Jalali Samina, Shami Sajjad Aslam, Ahmad Laiq, Batool Sajida, Kalsoom Ommia
Faculty of Biological Sciences, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Toxicol Lett. 2009 Oct 8;190(1):81-5. doi: 10.1016/j.toxlet.2009.07.003. Epub 2009 Jul 10.
Present study examined the genotoxic effects of arsenite in ovarian tissue of rat at 56 days of age. Immature (28 days old) female rats were exposed to different doses (50, 100, and 200 ppm) of sodium arsenite in drinking water for 28 days. DNA damage in ovarian tissue was measured by comet assay. All doses induced significant decrease in ovarian weight in a dose-dependent manner compared to control, more prominently at (P<0.001) 100 and 200 ppm. All the comet assay parameters showed significant difference with arsenite treatment compared to control group. In treatment groups, mean number of cells with intact DNA decreased while, mean comet number increased (P<0.001) in a dose-dependent manner compared to control. Significant decrease (P<0.05) was observed in mean comet length, height, comet head diameter and %DNA in comet head of high dose groups compared to control group. Dose dependent increase was found in mean comet tail length, %DNA in tail, tail moment and olive tail moment in high dose groups compared to control group. The study indicates that arsenic caused DNA damage to ovarian cells particularly at high doses and ensure comet assay as an effective method to detect DNA damage in tissue caused by metals.
本研究检测了亚砷酸盐对56日龄大鼠卵巢组织的遗传毒性作用。将未成熟（28日龄）雌性大鼠置于含不同剂量（50、100和200 ppm）亚砷酸钠的饮用水中，持续暴露28天。采用彗星试验检测卵巢组织中的DNA损伤。与对照组相比，所有剂量均以剂量依赖性方式导致卵巢重量显著下降，在100和200 ppm时更为明显（P<0.001）。与对照组相比，所有彗星试验参数在亚砷酸盐处理组均有显著差异。在处理组中，与对照组相比，DNA完整的细胞平均数减少，而彗星平均数呈剂量依赖性增加（P<0.001）。与对照组相比，高剂量组的平均彗星长度、高度、彗星头部直径和彗星头部DNA百分比均显著降低（P<0.05）。与对照组相比，高剂量组的平均彗尾长度、彗尾DNA百分比、尾矩和橄榄尾矩呈剂量依赖性增加。该研究表明，砷对卵巢细胞造成DNA损伤，尤其是在高剂量时，并证实彗星试验是检测金属所致组织DNA损伤的有效方法。
