Suetsugu Y, Saeki K, Matsubara H
Department of Biology, Faculty of Science, Osaka University, Japan.
FEBS Lett. 1991 Nov 4;292(1-2):13-6. doi: 10.1016/0014-5793(91)80822-k.
Plasmids which contained the translational fusion of Escherichia coli lacZ to Rhodobacter capsulatus ferredoxin genes, fdxN and fdxA, were constructed. Effects of growth conditions on the expression of each ferredoxin were analyzed by measuring the beta-galactosidase activity in R. capsulatus which harbored a corresponding plasmid. Transcription of fdxN::lacZ, the ferredoxin I fusion gene, was regulated at least 100-fold by either NH4+ or O2 but not by illumination, confirming that fdxN belongs to the nif-gene family. Transcription of fdxA::lacZ, the ferredoxin II fusion gene, however, was constant under all the conditions surveyed, suggesting that the protein has some constitutive function(s).
构建了含有大肠杆菌lacZ与荚膜红细菌铁氧化还原蛋白基因fdxN和fdxA翻译融合体的质粒。通过测量携带相应质粒的荚膜红细菌中的β-半乳糖苷酶活性，分析了生长条件对每种铁氧化还原蛋白表达的影响。铁氧化还原蛋白I融合基因fdxN::lacZ的转录受NH4+或O2调控至少100倍，但不受光照调控，这证实fdxN属于固氮基因家族。然而，铁氧化还原蛋白II融合基因fdxA::lacZ的转录在所有检测条件下都是恒定的，这表明该蛋白具有一些组成型功能。
