Miyamoto E, Hideshima Y, Teshima Y, Yamazaki R, Kakiuchi S
J Cyclic Nucleotide Res. 1977 Apr;3(2):85-94.
Both protein kinase modulator and phosphodiesterase activator activities were present in the supernatant fluid of a homogenate of bovine brain. These were separated on a DEAE-cellulose column chromatography. Separation was also achieved by an isoelectrofocusing fractionation of the supernatant fluid, isoelectric points of proteins kinase modulator and phospodiesterase activator being 4.25 and 4.44, respectively. Phospodiesterase activator was purified from bovine brain to an apparent homogenity by a procedure which did not involve a drastic treatment such as boiling. The purification of phosphodiesterase activator resulted in removing the protein kinase modulator activity and the ratio of the activity of protein kinase modulator to that of phosphodiesterase activator in the sample decreased as the purification proceeded.
蛋白激酶调节剂和磷酸二酯酶激活剂活性均存在于牛脑匀浆的上清液中。它们通过DEAE - 纤维素柱色谱进行分离。通过对上清液进行等电聚焦分级分离也实现了分离，蛋白激酶调节剂和磷酸二酯酶激活剂的等电点分别为4.25和4.44。通过一种不涉及如煮沸等剧烈处理的方法，从牛脑中纯化磷酸二酯酶激活剂至表观均一性。磷酸二酯酶激活剂的纯化导致蛋白激酶调节剂活性的去除，并且随着纯化的进行，样品中蛋白激酶调节剂活性与磷酸二酯酶激活剂活性的比率降低。
