Loura Luís M S, Prieto Manuel
Universidade de Coimbra, Rua do Norte, Coimbra 3000-295, Portugal.
Protein Pept Lett. 2009;16(7):726-35. doi: 10.2174/092986609788681698.
Interaction of fusogenic or membrane-perturbing peptides with lipid bilayers often involves drastic rearrangements of the membrane structure, with redistribution of lipids, inducement of bilayer curvature, or formation of non-bilayer or multibilayer structures. Fluorescence (or Förster) Resonance Energy Transfer (FRET) is a photophysical technique which has an acute sensitivity to distances in the nanometer range, and, as such, is particularly suited to probe alterations in membrane organization in this length scale. This article reviews methods and selected applications of FRET in this field, from the now classic (fusion induced) lipid-mixing assay to examples where kinetic modeling of FRET enables the recovery of topological information.
促融肽或膜扰动肽与脂质双层的相互作用通常涉及膜结构的剧烈重排，包括脂质的重新分布、双层曲率的诱导或非双层或多层结构的形成。荧光（或福斯特）共振能量转移（FRET）是一种对纳米范围内的距离具有敏锐灵敏度的光物理技术，因此特别适合探测该长度尺度下膜组织的变化。本文综述了FRET在该领域的方法和选定应用，从现在经典的（融合诱导的）脂质混合测定到FRET动力学建模能够恢复拓扑信息的示例。
