PIQA：Illumina G1 基因组分析仪数据质量评估流水线。
PIQA: pipeline for Illumina G1 genome analyzer data quality assessment.
机构信息
Department of Computer Science, University of Houston, Houston, TX, USA.
出版信息
Bioinformatics. 2009 Sep 15;25(18):2438-9. doi: 10.1093/bioinformatics/btp429. Epub 2009 Jul 14.
SUMMARY
PIQA is a quality analysis pipeline designed to examine genomic reads produced by Next Generation Sequencing technology (Illumina G1 Genome Analyzer). A short statistical summary, as well as tile-by-tile and cycle-by-cycle graphical representation of clusters density, quality scores and nucleotide frequencies allow easy identification of various technical problems including defective tiles, mistakes in sample/library preparations and abnormalities in the frequencies of appearance of sequenced genomic reads. PIQA is written in the R statistical programming language and is compatible with bustard, fastq and scarf Illumina G1 Genome Analyzer data formats.
AVAILABILITY
The PIQA pipeline, installation instructions and examples are available at the supplementary web site (http://bioinfo.uh.edu/PIQA).
摘要
PIQA 是一个质量分析管道，旨在检查下一代测序技术（Illumina G1 基因组分析仪）产生的基因组读取。简短的统计摘要，以及聚类密度、质量得分和核苷酸频率的逐块和逐循环图形表示，可轻松识别各种技术问题，包括有缺陷的瓦片、样品/库制备中的错误以及测序基因组读取出现频率的异常。PIQA 是用 R 统计编程语言编写的，与 bustard、fastq 和 scarf Illumina G1 基因组分析仪数据格式兼容。
可用性
PIQA 管道、安装说明和示例可在补充网站上获得（http://bioinfo.uh.edu/PIQA）。
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