Ziegler R J, Pozos R S
J Neuropathol Exp Neurol. 1977 Jul;36(4):680-92. doi: 10.1097/00005072-197707000-00004.
The ultrastructural consequences of herpes simplex virus type 2 (strain R-2) infection of organotypic cultures of embryonic rat dorsal root ganglia were studied. The intial consequences (dilation of endoplasmic reticulum and/or Golgi apparatus and distortion of mitochondrial structure) occurred in the cytoplasm. These effects were followed by several nuclear changes including loss of nucleoplasm, and margination of chromatin. Extensive nuclear membrane proliferation was accompanied by the viral maturation process. Two previously unreported observations were made. First, productive virus replications occurred in glial cells, as well as in neurons. Mature, enveloped virus was produced by nuclear budding and envelopment in the cytoplasm in both cell types. Second, neurons were observed to participate in polykaryocyte formation with other neurons and with glial cells. These polykaryocytes were usually composed of only three or four cells. Neuronal-glial polykaryocytes were more prevalent than neuronal-neuronal polykaryocytes. In general, however, the ultrastructural changes in neurons and glial cells in culture were consistent with previously reported changes occurring in nervous tissue of experimental animals suffering from acute herpes simplex virus infections. Therefore, the utilization of this in vitro system to further study the pathogenesis of acute herpetic infections of sensory ganglia appears to be reasonable.
对胚胎大鼠背根神经节器官型培养物进行单纯疱疹病毒2型（R - 2株）感染后的超微结构变化进行了研究。最初的变化（内质网和/或高尔基体扩张以及线粒体结构扭曲）发生在细胞质中。这些效应之后出现了一些核变化，包括核质丧失和染色质边缘化。广泛的核膜增殖伴随着病毒成熟过程。有两个以前未报道过的观察结果。第一，有活性的病毒复制发生在神经胶质细胞以及神经元中。在这两种细胞类型中，成熟的包膜病毒都是通过核出芽和在细胞质中包裹形成的。第二，观察到神经元与其他神经元以及神经胶质细胞一起参与多核细胞的形成。这些多核细胞通常仅由三四个细胞组成。神经胶质 - 神经元多核细胞比神经元 - 神经元多核细胞更普遍。然而，一般来说，培养物中神经元和神经胶质细胞的超微结构变化与先前报道的急性单纯疱疹病毒感染实验动物神经组织中发生的变化一致。因此，利用这种体外系统进一步研究感觉神经节急性疱疹感染的发病机制似乎是合理的。
