Hartmann M A, Ziegler R J
J Neuropathol Exp Neurol. 1979 Mar;38(2):165-76. doi: 10.1097/00005072-197903000-00007.
An in vitro model system was developed to study the effects that immune cells might have on herpes simplex virus (HSV) infection of rat dorsal root ganglia in culture. Our results demonstrate that rat splenic lymphocytes and peritoneal exudate cells are incapable of replicating HSV even if these cells come from sensitized animals and are stimulated in vitro. Both cell preparations can offer some protection to rat dorsal root ganglia from the effects of HSV infection. The data suggest that an immunologically non-specific (not mediated by sensitized cells) type of protection is important to neurons, while an immunologically specific (mediated by sensitized cells) protection is most beneficial to fibroblasts. This system can be utilized to study the mechanism of latency since the neurons of sensory ganglia are the natural site of latent herpes virus.
我们建立了一个体外模型系统，以研究免疫细胞可能对培养的大鼠背根神经节单纯疱疹病毒（HSV）感染产生的影响。我们的结果表明，大鼠脾淋巴细胞和腹腔渗出细胞即使来自致敏动物并在体外受到刺激，也无法复制HSV。两种细胞制剂都可以为大鼠背根神经节提供一定保护，使其免受HSV感染的影响。数据表明，一种免疫非特异性（不由致敏细胞介导）的保护类型对神经元很重要，而免疫特异性（由致敏细胞介导）的保护对成纤维细胞最有益。由于感觉神经节的神经元是潜伏性疱疹病毒的天然位点，因此该系统可用于研究潜伏期的机制。
