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合成并鉴定包含组氨酸 1 和 P2Y(1)受体的细胞膜亲和层析柱：一种多 G 蛋白偶联受体柱。

Synthesis and characterization of a cellular membrane affinity chromatography column containing histamine 1 and P2Y(1) receptors: a multiple G-protein coupled receptor column.

机构信息

National Institute on Aging, National Institutes of Health, Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, United States.

出版信息

J Pharm Biomed Anal. 2010 Jul 8;52(3):416-9. doi: 10.1016/j.jpba.2009.06.035. Epub 2009 Jun 26.

DOI:10.1016/j.jpba.2009.06.035
PMID:19608372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4605401/
Abstract

A cellular membrane affinity chromatography (CMAC) column has been created using cellular membrane fragments from a 1321N1 cell line stably transfected with the P2Y(1) receptor. The CMAC(1321N1(P2Y1)) column contained functional P2Y(1) and histamine 1 receptors, which independently bound receptor-specific ligands. The data obtained with the CMAC(1321N1(P2Y1)) column demonstrate that multiple-G-protein coupled receptor (GPCR) columns can be developed and used to probe interactions with the immobilized receptors and that endogenously expressed GPCRs can be used to create CMAC columns. The results also establish that the histamine 1 receptor can be immobilized with retention of ligand-specific binding.

摘要

已使用通过 P2Y(1) 受体稳定转染的 1321N1 细胞系的细胞膜碎片创建了细胞表面亲合色谱 (CMAC) 柱。CMAC(1321N1(P2Y1)) 柱含有功能 P2Y(1) 和组胺 1 受体，它们可分别与受体特异性配体结合。使用 CMAC(1321N1(P2Y1)) 柱获得的数据表明，可以开发和使用多- G 蛋白偶联受体 (GPCR) 柱来探测与固定化受体的相互作用，并且可以使用内源性表达的 GPCR 来创建 CMAC 柱。结果还表明，组胺 1 受体可以固定化而保留配体特异性结合。

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