Azizi Ali, Sirskyj Danylo, Weltzin Richard, Anderson David E, Diaz-Mitoma Francisco
Department of Pathology and Laboratory Medicine, University of Ottawa, 451 Smyth, Ottawa, ON K1H 8M5, Canada.
Infectious Disease and Vaccine Research Center, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth, Ottawa, ON K1H 8L1, Canada.
J Med Microbiol. 2009 Nov;58(Pt 11):1433-1436. doi: 10.1099/jmm.0.012203-0. Epub 2009 Jul 16.
The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.
甲型肝炎病毒（HAV）在细胞培养中生长缓慢，这是开发灵敏快速的HAV及相关中和抗体检测和定量方法的主要障碍之一。目前，体外检测通常需要8天或更长时间来检测和定量HAV中和抗体的存在。本研究描述了一种快速免疫测定法，该方法仅需3天就能检测出抗HAV中和抗体。这种基于微孔板的酶法测定可能适用于病毒学诊断、评估HAV疫苗的免疫原性以及在HAV感染过程中定量中和抗体。
