埃博拉病毒糖蛋白抗原模拟物的设计与合成
Design and synthesis of an antigenic mimic of the Ebola glycoprotein.
作者信息
Rutledge Ryan D, Huffman Brian J, Cliffel David E, Wright David W
机构信息
Vanderbilt University, Department of Chemistry, Nashville, Tennessee 37235.
出版信息
J Mater Res. 2008;23(12):3161-3168. doi: 10.1557/JMR.2008.0384.
Abstract
An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (K(d) = 12 × 10(-6) M) within two orders of magnitude of the reported affinity of the native Ebola glycoprotein for the same antibody. These results suggest that the rational design approach described herein is a suitable method for the further development of protein-based antigenic mimics with potential applications in vaccine development and sensor technology.
摘要
合成了埃博拉病毒糖蛋白的一种抗原模拟物，并测试了其被抗埃博拉病毒糖蛋白抗体识别的能力。表位作图程序产生了一个合适的表位，当该表位呈现在纳米颗粒表面时，形成一种能被针对天然蛋白的抗体识别的结构。这种模拟物与抗体的相互作用已通过基于酶联免疫吸附测定（ELISA）和石英晶体微天平（QCM）的方法进行了定量，得到的亲和力（解离常数K(d)=12×10⁻⁶ M）与报道的天然埃博拉病毒糖蛋白对同一抗体的亲和力在两个数量级范围内。这些结果表明，本文所述的合理设计方法是进一步开发基于蛋白质的抗原模拟物的合适方法，这些模拟物在疫苗开发和传感器技术中具有潜在应用。
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