经（-）-氯甲酸薄荷酯衍生化后，采用气相色谱/质谱法检测尿液中的苯丙胺和甲基苯丙胺。
Detection of amphetamine and methamphetamine in urine by gas chromatography/mass spectrometry following derivatization with (-)-menthyl chloroformate.
作者信息
Hughes R O, Bronner W E, Smith M L
机构信息
Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
出版信息
J Anal Toxicol. 1991 Sep-Oct;15(5):256-9. doi: 10.1093/jat/15.5.256.
A gas chromatography/mass spectrometry assay was developed for the detection of amphetamine and methamphetamine in urine. These drugs were detected as carbamate derivatives following reaction with (-)-menthyl chloroformate. Selected ion monitoring of the 226, 182, and 227 dalton ions for amphetamine and the 240, 102, and 196 dalton ions for methamphetamine provided positive analyte identification. Quantitative values obtained by isotope dilution mass spectrometry displayed a linear response from below 50 to above 6,000 ng/mL. Differentiation of over-the-counter (R)-methamphetamine from illicit (S)-methamphetamine was accomplished by chromatographic separation of the diastereomeric derivatives on an achiral column. No interferences were observed from ephedrine, pseudoephedrine, phentermine, phenylpropanolamine, or 2-phenylethylamine.
开发了一种气相色谱/质谱分析法用于检测尿液中的苯丙胺和甲基苯丙胺。与（-）-薄荷基氯甲酸酯反应后，这些药物被检测为氨基甲酸酯衍生物。对苯丙胺的226、182和227道尔顿离子以及甲基苯丙胺的240、102和196道尔顿离子进行选择离子监测，可实现对分析物的阳性鉴定。通过同位素稀释质谱法获得的定量值在低于50至高于6000 ng/mL范围内呈线性响应。通过在手性柱上对非对映体衍生物进行色谱分离，实现了非处方（R）-甲基苯丙胺与非法（S）-甲基苯丙胺的区分。未观察到麻黄碱、伪麻黄碱、芬特明、苯丙醇胺或2-苯乙胺的干扰。
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