Department of Pediatrics, East West Kidney Diseases Research Institute, Kyung Hee University, Seoul, Korea.
Phytother Res. 2010 Mar;24(3):339-43. doi: 10.1002/ptr.2936.
This study aimed to investigate the inhibitory effect of Bupleurum falcatum and its combination with angiotensin II receptor blocker (ARB) on cytokine and chemokine production in cultured human mesangial cells. Human mesangial cells were isolated and cultured in Dulbecco's modified Eagle's medium culture medium. Bupleurum falcatum, ARB, and the combination of the two were added to human mesangial cells. Cytokine and chemokine levels were analysed using an enzyme-linked immunosorbent assay. There were no significant differences in the expression of IL-1ss, IL-2 or TNF-a between controls and the experimental groups. However, IL-11 and monocyte chemoattractant protein-1 (MCP-1) levels were significantly reduced in response to ARB, Bupleurum falcatum, or their combination when compared with controls. IL-8 expression was reduced significantly only in cells treated with ARB. Both Bupleurum falcatum and ARB treatments alone reduced the cytokine concentration, but there was not a stronger reduction when the two drugs were combined. It was shown that Bupleurum falcatum inhibited cytokine production in human mesangial cells. However, there were no additive effects on the suppression of cytokine production when Bupleurum falcatum was combined with ARB. Further studies are needed to elucidate the renoprotective effects of Bupleurum falcatum.
本研究旨在探讨柴胡及其与血管紧张素 II 受体阻滞剂 (ARB) 联合应用对培养的人肾小球系膜细胞细胞因子和趋化因子产生的抑制作用。人肾小球系膜细胞用 Dulbecco 改良 Eagle 培养基培养并分离培养。向人肾小球系膜细胞中加入柴胡、ARB 和两者的组合。用酶联免疫吸附试验分析细胞因子和趋化因子水平。与对照组相比，IL-1ss、IL-2 或 TNF-a 的表达在实验组之间没有显著差异。然而，与对照组相比，ARB、柴胡或两者的组合可显著降低 IL-11 和单核细胞趋化蛋白-1 (MCP-1) 的水平。仅在 ARB 处理的细胞中，IL-8 的表达显著降低。柴胡和 ARB 单独治疗均可降低细胞因子浓度，但两者联合使用时并未增强抑制作用。结果表明，柴胡抑制人肾小球系膜细胞细胞因子的产生。然而，当柴胡与 ARB 联合使用时，对细胞因子产生的抑制作用没有相加作用。需要进一步的研究来阐明柴胡的肾保护作用。
