Miner-Williams Warren, Moughan Paul J, Fuller Malcolm F
Riddet Institute, Massey University, Palmerston North, New Zealand.
J Agric Food Chem. 2009 Jul 22;57(14):6029-35. doi: 10.1021/jf901036r.
The aim was to compare five techniques commonly used to quantify mucin concentrations in ileal digesta collected from three growing pigs that had been fed a diet in which the sole protein was casein. Ileal mucin output was estimated by the periodic acid-Schiff, ethanol precipitation, and phenol-sulfuric acid methods as 25.1, 19.3, and 20.7 g kg-1 of dry matter intake (DMI), respectively. The mucin concentration estimated from sialic acid was only 5.9 g kg-1 of DMI. On the basis of the concentrations of the hexosamines N-acetylglucosamine and N-acetylgalactosamine, mucin output was estimated as 44.9 g kg-1 pf DMI. Of the five assays studied, the ethanol precipitation, periodic acid-Schiff, phenol-sulfuric acid, and sialic acid methods may considerably underestimate mucin in the digesta, which calls into question the accuracy of all of these approaches. In contrast, the gas chromatography method for the determination of hexosamines gave more information on the type and state of the mucin present.
目的是比较五种常用于定量分析从三头生长猪收集的回肠食糜中黏蛋白浓度的技术，这些猪食用的日粮中唯一的蛋白质是酪蛋白。通过高碘酸 - 席夫反应法、乙醇沉淀法和苯酚 - 硫酸法估计回肠黏蛋白输出量分别为每千克干物质摄入量（DMI）25.1、19.3和20.7克。根据唾液酸估计的黏蛋白浓度仅为每千克DMI 5.9克。根据己糖胺N - 乙酰葡糖胺和N - 乙酰半乳糖胺的浓度，黏蛋白输出量估计为每千克DMI 44.9克。在所研究的五种测定方法中，乙醇沉淀法、高碘酸 - 席夫反应法、苯酚 - 硫酸法和唾液酸法可能会大大低估食糜中的黏蛋白，这使得所有这些方法的准确性受到质疑。相比之下，用于测定己糖胺的气相色谱法能提供更多关于所存在黏蛋白的类型和状态的信息。
