Taormina P J, Dorsa W J
Corporate Food Safety, John Morrell & Co., Cincinnati, Ohio 45246, USA.
J Food Prot. 2009 Jun;72(6):1216-24. doi: 10.4315/0362-028x-72.6.1216.
The antimicrobial lauric arginate (LAE) alone or in combination with an antimicrobial liquid smoke extract was studied as a postlethality treatment against Listeria monocytogenes on vacuum-packaged frankfurters. Treatment with 2 ml of 5,000 ppm of LAE reduced L. monocytogenes from 7.13 to 5.82 log CFU per package on day 0, and treatment with 3 ml reduced populations from 7.22 to 5.79 log CFU per package on day 0. Treatment with LAE and smoke flavor did not result in significantly different populations of L. monocytogenes (P < 0.05) compared with LAE alone. Log reductions on frankfurters 48 h after treatment with 3 to 4.5 ml of 5000 ppm of LAE ranged from 1.80 to 2.22, but these values were not significantly different between treatment volumes. Populations on dip-inoculated frankfurters 1 h after spraying with 2.5 ml of water (control) were 7.69 log CFU per package, and frankfurters treated with 2.5 ml of 5000 or 8000 ppm of LAE had populations of 6.03 and 5.85 log CFU per package, respectively. On spot-inoculated frankfurters, spray treatment with 2.5 ml of water resulted in L. monocytogenes counts of 8.16 log CFU per package after 1 h, whereas spray treatment with LAE at 5000 and 8000 ppm reduced counts within 1 h to 6.80 and 6.13 log CFU per package, respectively. All treatments resulted in reductions of > or = 1.68 log CFU per package, and many of the treatments caused a > 2-log reduction within 48 h. In this study, the shortterm efficacy of LAE against L. monocytogenes on vacuum-packaged frankfurters was demonstrated, providing support for use of this postlethality treatment on frankfurters and sausages for control of this pathogen.
研究了单独使用抗菌剂月桂酰精氨酸（LAE）或其与抗菌性液态烟熏提取物联合使用，作为对真空包装法兰克福香肠上的单核细胞增生李斯特菌的致死后处理方法。用2毫升5000 ppm的LAE处理，在第0天可使每包法兰克福香肠中的单核细胞增生李斯特菌数量从7.13 log CFU降至5.82 log CFU，用3毫升处理可使每包数量从7.22 log CFU降至5.79 log CFU。与单独使用LAE相比，LAE与烟熏风味剂联合处理的单核细胞增生李斯特菌数量没有显著差异（P < 0.05）。用3至4.5毫升5000 ppm的LAE处理48小时后，法兰克福香肠上的对数减少量在1.80至2.22之间，但不同处理体积之间的这些值没有显著差异。用2.5毫升水（对照）喷洒后1小时，浸入接种的法兰克福香肠上的菌数为每包7.69 log CFU，用2.5毫升5000 ppm或8000 ppm的LAE处理的法兰克福香肠上的菌数分别为每包6.03和5.85 log CFU。对于点接种的法兰克福香肠，用2.5毫升水喷雾处理1小时后，每包单核细胞增生李斯特菌计数为8.16 log CFU，而用5000 ppm和8000 ppm的LAE喷雾处理在1小时内分别将计数降至每包6.80和6.13 log CFU。所有处理均导致每包减少≥1.68 log CFU，许多处理在48小时内导致减少>2个对数。在本研究中，证明了LAE对真空包装法兰克福香肠上的单核细胞增生李斯特菌具有短期有效性，为在法兰克福香肠和香肠上使用这种致死后处理方法来控制这种病原体提供了支持。
