Dinu Laura-Dorina, Delaquis Pascal, Bach Susan
Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97 South, Summerland, British Columbia, Canada.
J Food Prot. 2009 Jun;72(6):1342-54. doi: 10.4315/0362-028x-72.6.1342.
Concerns about animal enteropathogen contamination of fresh horticultural products have,increased worldwide and are mainly due to the ability of bacteria to survive under stress conditions in the agricultural environment and during raw-vegetable processing. This review challenges the idea that the viable but nonculturable phenomenon that has been proven to occur in plant-associated environments contributes to human pathogen survival and might be correlated with foodborne infection. Factors associated with the nonculturable response of bacteria in the field and during postharvest processing and distribution are discussed, specifically for the most common animal enteropathogens linked with the consumption of raw products: Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Shigella spp. The accurate detection of live bacterial populations is essential for pathogen screening in food and environmental safety control and in epidemiological analysis and may have to be considered for identification of critical control points at the time of food inspection.
全球范围内，对新鲜园艺产品受动物肠道病原体污染的担忧日益增加，这主要是因为细菌有能力在农业环境以及生鲜蔬菜加工过程中的应激条件下存活。本综述对以下观点提出了质疑：已证实在植物相关环境中出现的活的但不可培养现象会促进人类病原体存活，并且可能与食源性感染相关。本文讨论了在田间以及收获后加工和分销过程中，与细菌不可培养反应相关的因素，特别是针对与生食消费相关的最常见动物肠道病原体：大肠杆菌O157:H7、沙门氏菌、单核细胞增生李斯特菌和志贺氏菌属。准确检测活菌数量对于食品和环境安全控制以及流行病学分析中的病原体筛查至关重要，并且在食品检查时确定关键控制点时可能也需要考虑。
