采用高效薄层色谱法检测黄芩原材料及提取物中潜在的掺假物石蚕叶委陵菜。

Detection of the potential adulterant Teucrium chamaedrys in Scutellaria baicalensis raw material and extract by high-performance thin-layer chromatography.

作者信息

Hong Thao, Jeong Mijeong Lee, Zahn Michael, Fay Brooke A, Lee Kangwoo, Hwangbo Huunjung, Park Eunhye, Kim Minjung, Ma Wenwen

机构信息

Unigen Inc., Quality Control/Quality Assurance Department, 2660 Willamette Dr, NE, Lacey, WA 98516, USA.

出版信息

J AOAC Int. 2009 May-Jun;92(3):785-8.

PMID:19610368

Abstract

Teucrium chamaedrys (Gemander) has been reported as an adulterant of Scutellaria lateriflora (American skullcap) herbal preparations and is also known to be hepatotoxic. A quick and simple high-performance thin-layer chromatographic (HPTLC) method was developed for the detection of T. chamaedrys (Germander) in S. baicalensis (Chinese skullcap) extract, an ingredient of the proprietary blend product, Univestin. The HPTLC profile of T. chamaedrys was distinguished from that of S. baicalensis by its bright green fluorescence bands. This simple method can be completed in an hour for the quality control of Univestin and its raw material, S. baicalensis. The method is sensitive and can detect T. chamaedrys at levels as low as 0.5% (w/w).

摘要

香科科已被报道为广布黄芩草药制剂的掺假物，并且已知具有肝毒性。开发了一种快速简便的高效薄层色谱（HPTLC）方法，用于检测专有混合产品Univestin的成分黄芩提取物中的香科科。香科科的HPTLC图谱通过其亮绿色荧光带与黄芩的图谱区分开来。这种简单的方法可在一小时内完成，用于Univestin及其原料黄芩的质量控制。该方法灵敏，能够检测低至0.5%（w/w）水平的香科科。