Nowak Monika, Wyszko Eliza, Fedoruk-Wyszomirska Agnieszka, Pospieszny Henryk, Barciszewska Mirosława Z, Barciszewski Jan
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
FEBS J. 2009 Aug;276(16):4372-80. doi: 10.1111/j.1742-4658.2009.07145.x. Epub 2009 Jul 15.
We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.
我们在此报告一种由双链DNA诱导的抑制RNA病毒的新方法。我们证明，长双链DNA分子以及与病毒RNA序列互补的短干扰DNA均能抑制烟草花叶病毒的表达并阻止病毒传播。此外，HeLa细胞中HIV-1 gp41基因的表达也受到互补短干扰DNA的抑制。我们发现Dicer可处理双链DNA，这表明参与RNA沉默的细胞机制被激活。对于由双链DNA实现的病毒RNA沉默，我们创造了“DNA干扰技术”这一术语。
