INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
J Chem Neuroanat. 2009 Dec;38(4):257-65. doi: 10.1016/j.jchemneu.2009.07.004. Epub 2009 Jul 15.
The vagal motor system is involved in the regulation of cardiorespiratory and gastrointestinal functions. Vagal motor neurons are localized near or adjacent to catecholaminergic neurons, but their co-localisation seems species dependent, present in the cat but absent in the rabbit. In pig, a species commonly used as an experimental model in humans brain disorders (sudden infant death syndrome, hypoxia), the relationship is poorly understood. We aimed at describing the distribution of vagal motor neurons and tyrosine hydroxylase-immunoreactive (-ir) neurons by using a double staining method in combination with retrograde tracing of vagal efferent neurons. After fluorogold impregnation of the central part of the sectioned left cervical vagal trunk, two main vagal motor neuronal populations were located in the dorsal motor nucleus of the vagus nerve (DMX) and in the area of the nucleus ambiguus (Amb). Like in the human, the DMX was composed of different subpopulations of neurons with the same morphological characteristics. Immunohistochemistry of catecholaminergic synthesizing enzymes differentiated two main sites containing vagal motor populations: the dorsomedial and the ventrolateral medulla. TH-ir was rarely seen in vagal motor neurons of the DMX, but TH-ir neurons were present around the two main vagal motor neuronal populations that contained TH-ir fibres. The anatomical organisation of the vagal motor and the catecholaminergic neuronal systems are similar to those described in humans and suggest that the involvement of the catecholamines in the control of the vagal motor system may be similar in pigs and in humans.
迷走运动系统参与了心肺和胃肠道功能的调节。迷走运动神经元位于儿茶酚胺能神经元附近或与之相邻，但它们的共定位似乎依赖于物种，在猫中存在，但在兔中不存在。在猪中，作为人类脑部疾病（婴儿猝死综合征、缺氧）实验模型的常用物种，这种关系尚不清楚。我们旨在通过逆行追踪迷走传出神经元的双重染色方法，描述迷走运动神经元和酪氨酸羟化酶免疫反应性（-ir）神经元的分布。在左侧颈迷走神经干的节段性中央部分进行氟金胺浸渍后，两个主要的迷走运动神经元群位于迷走神经背核（DMX）和疑核（Amb）区域。与人类一样，DMX 由具有相同形态特征的不同神经元亚群组成。儿茶酚胺合成酶的免疫组织化学将含有迷走运动神经元的两个主要部位区分开来：背内侧和腹外侧髓质。TH-ir 在 DMX 的迷走运动神经元中很少见，但在含有 TH-ir 纤维的两个主要迷走运动神经元群周围存在 TH-ir 神经元。迷走运动和儿茶酚胺能神经元系统的解剖组织与在人类中描述的相似，表明儿茶酚胺在迷走运动系统控制中的参与在猪和人类中可能相似。
