Laiwattanapaisal Wanida, Kunanuvat Usakorn, Intharachuti Wichabhorn, Chinvongamorn Chakorn, Hannongbua Supot, Chailapakul Orawon
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Talanta. 2009 Sep 15;79(4):1104-10. doi: 10.1016/j.talanta.2009.02.020. Epub 2009 Feb 20.
A simple, specific and sensitive sequential injection analysis (SIA) system based on non-immunoassay fluorescent detection has been developed for the determination of urinary albumin. The specific binding of the dye Albumin Blue 580 (AB 580) to albumin in urine generated high emission fluorescent signals. The excitation and emission wavelengths were set at 590 and 610 nm, respectively. The analytical range was obtained from 1 to 100 mg L(-1), with a detection limit of 0.3 mg L(-1) (S/N=3). The SIA system gave high precision with relative standard deviations (R.S.D.s) of 0.9% and 1.4% when evaluated with 15 and 100 mg L(-1) albumin (n=15), respectively. The method exhibited good reproducibility, as assessed by performing four analytical curves on different days, and intra-run CVs (2.3-3.3%) and inter-run CVs (3.8%) were obtained. Rapid operation was achieved with a sample throughput of 37 h(-1). This method was successfully applied to the determination of urinary albumin, and the method was highly correlated with the immunoturbidimetric method (r(2)=0.965; n=72).
已开发出一种基于非免疫分析荧光检测的简单、特异且灵敏的顺序注射分析（SIA）系统，用于测定尿白蛋白。染料白蛋白蓝580（AB 580）与尿液中的白蛋白特异性结合，产生高发射荧光信号。激发波长和发射波长分别设定为590和610 nm。分析范围为1至100 mg L(-1)，检测限为0.3 mg L(-1)（S/N=3）。当用15和100 mg L(-1)白蛋白评估时（n=15），SIA系统具有高精度，相对标准偏差（R.S.D.s）分别为0.9%和1.4%。通过在不同日期绘制四条分析曲线评估，该方法具有良好的重现性，批内变异系数（CVs）为2.3 - 3.3%，批间变异系数为3.8%。样品通量为37 h(-1)，实现了快速操作。该方法成功应用于尿白蛋白的测定，且与免疫比浊法高度相关（r(2)=0.965；n=72）。
