Institute of Environmental Engineering, National Chiao Tung University, Hsinchu 300, Taiwan, ROC.
J Hazard Mater. 2009 Nov 15;171(1-3):1112-9. doi: 10.1016/j.jhazmat.2009.06.130. Epub 2009 Jun 30.
Anaerobic biodegradation of phenanthrene (PHE) was investigated using an enrichment culture consists predominantly of sulfate-reducing bacteria (87+/-6%). Aqueous biodegradation experiments were designed using the rotatable central composite design with five levels. The designed concentrations were 2-50 mg L(-1) for PHE, 480-3360 mg L(-1) for sulfate, and 5-50 mg L(-1) for initial biomass. Experimental results indicated that the biomass concentration was the most significant variable, followed by the sulfate and PHE concentrations. The desirability functions methodology (DFM) was applied to find out the maximum specific PHE removal rate (R(s)). The maximum R(s) of 9.0 mg g(-1)VSS d(-1) within the designed ranges was obtained when the initial PHE, sulfate and biomass concentrations were 18.5, 841 and 50 mg L(-1), respectively. The R(s) observed in the present study was higher than the values reported in the previous studies. Subsequently, a confirmation study was performed under the optimal conditions, and the results matched well with the R(s) estimated using DFM. Samples collected during PHE biodegradation experiments inferred the formation of two novel metabolic intermediates, 2-methyl-5-hydroxybenzaldehyde and 1-propenyl-benzene, and subsequently degraded to p-cresol, phenol and hydrocarbons.
采用主要由硫酸盐还原菌（87+/-6%）组成的富集培养物研究了菲（PHE）的厌氧生物降解。使用具有五个水平的可旋转中心组合设计进行了水相生物降解实验。设计的浓度为 PHE 的 2-50mgL(-1)、硫酸盐的 480-3360mgL(-1)和初始生物量的 5-50mgL(-1)。实验结果表明，生物量浓度是最重要的变量，其次是硫酸盐和 PHE 浓度。应用可接受性函数方法（DFM）找出最大特定 PHE 去除率（R(s)）。当初始 PHE、硫酸盐和生物量浓度分别为 18.5、841 和 50mgL(-1)时，在设计范围内获得了 9.0mg g(-1)VSS d(-1)的最大 R(s)。本研究中观察到的 R(s)高于以前研究中报道的值。随后，在最佳条件下进行了确认研究，结果与使用 DFM 估计的 R(s)吻合良好。在 PHE 生物降解实验中收集的样品推断出两种新型代谢中间产物的形成，即 2-甲基-5-羟基苯甲醛和 1-丙烯基苯，随后降解为对甲酚、苯酚和碳氢化合物。
