Unidad de Investigación, Hospital de León, Spain.
Immunol Lett. 2009 Sep 22;126(1-2):16-21. doi: 10.1016/j.imlet.2009.07.006. Epub 2009 Jul 17.
Two of the better characterized antimicrobial mechanisms displayed by human neutrophils are the reactive oxygen species (ROS) production and the induction of apoptosis. Their importance in mycobacterial infections is, however, controversial and we aimed to analyze them simultaneously in neutrophils infected with either Mycobacterium tuberculosis or the non-pathogenic M. gordonae. Neither species is eliminated by neutrophils but the pattern exhibited for both activities is completely different. M. tuberculosis induces ROS production and apoptosis but M. gordonae does not. Additional evidence was provided by an attenuated strain of M. gordonae that, although it has become susceptible to the antimicrobial activity of neutrophils, it still does not promote ROS production or apoptosis. Therefore no relationship could be established between any of these activities and the ability of neutrophils to kill mycobacteria. We have also observed that neutrophil concentration, a variable that is important in the antimicrobial activity against other pathogens, has no influence in the mycobacterial intracellular growth.
中性粒细胞表现出的两种特征较为明显的抗微生物机制是活性氧（ROS）的产生和细胞凋亡的诱导。然而，它们在分枝杆菌感染中的重要性存在争议，我们旨在同时分析感染结核分枝杆菌或非致病性戈登分枝杆菌的中性粒细胞中的这两种机制。这两种分枝杆菌都不会被中性粒细胞清除，但这两种活性表现出的模式却完全不同。结核分枝杆菌诱导 ROS 的产生和细胞凋亡，但戈登分枝杆菌则不会。M. gordonae 的一种减毒菌株提供了额外的证据，尽管它对中性粒细胞的抗菌活性变得敏感，但它仍然不会促进 ROS 的产生或细胞凋亡。因此，这些活性与中性粒细胞杀死分枝杆菌的能力之间没有关系。我们还观察到，在针对其他病原体的抗菌活性中非常重要的一个变量，即中性粒细胞浓度，对分枝杆菌的细胞内生长没有影响。
