Advanced Medical Emergency and Critical Care Center, Yamaguchi University Hospital, Ube 755-8505, Japan.
Free Radic Biol Med. 2009 Oct 1;47(7):1039-48. doi: 10.1016/j.freeradbiomed.2009.07.012. Epub 2009 Jul 17.
The dynamics of superoxide anion (O(2)(-)) in vivo remain to be clarified because no appropriate method exists to directly and continuously monitor and evaluate O(2)(-) in vivo. Here, we establish an in vivo method using a novel electrochemical O(2)(-) sensor. O(2)(-) generated is measured as a current and evaluated as a quantified partial value of electricity (Q(part)), which is calculated by integration of the difference between the baseline and the actual reacted current. The accuracy and efficacy of this method were confirmed by dose-dependent O(2)(-) generation in xanthine-xanthine oxidase in vitro in phosphate-buffered saline and human blood. It was then applied to endotoxemic rats in vivo. O(2)(-) current began to increase 1 h after lipopolysaccharide, and Q(part) increased significantly for 6 h in endotoxemic rats, in comparison to sham-treated rats. These values were attenuated by superoxide dismutase. The generation and attenuation of O(2)(-) were indirectly confirmed by plasma lipid peroxidation with malondialdehyde, endothelial injury with soluble intercellular adhesion molecule-1, and microcirculatory dysfunction. This is a novel method for measuring O(2)(-) in vivo and could be used to monitor and treat the pathophysiology caused by excessive O(2)(-) generation in animals and humans.
超氧阴离子（O(2)(-))在体内的动态仍不清楚，因为没有适当的方法可以直接和连续地监测和评估体内的 O(2)(-)。在这里，我们使用一种新型的电化学 O(2)(-)传感器建立了一种体内方法。生成的 O(2)(-)被测量为电流，并评估为量化的部分电量 (Q(part))，这是通过整合基线和实际反应电流之间的差异来计算的。该方法的准确性和有效性通过在磷酸盐缓冲液和人血中的黄嘌呤-黄嘌呤氧化酶体外的剂量依赖性 O(2)(-)生成得到了证实。然后将其应用于体内内毒素血症大鼠。在脂多糖后 1 小时，O(2)(-)电流开始增加，内毒素血症大鼠的 Q(part)在 6 小时内显著增加，与假手术大鼠相比。这些值通过超氧化物歧化酶减弱。通过丙二醛的血浆脂质过氧化、可溶性细胞间黏附分子-1 的内皮损伤和微循环功能障碍间接证实了 O(2)(-)的产生和衰减。这是一种测量体内 O(2)(-)的新方法，可用于监测和治疗动物和人类中由过量 O(2)(-)生成引起的病理生理学。
