Wittner M, Di Stefano A, Mandon B, Roinel N, de Rouffignac C
Departement de Biologie Cellulaire et Moléculaire, Centre d'Etudes Saclay, Gif-sur-Yvette, France.
Pflugers Arch. 1991 Sep;419(2):212-4. doi: 10.1007/BF00373010.
The effect of antidiuretic hormone (ADH) on transepithelial Na+ Cl-, Ca2+ and Mg2+ net fluxes (JNa, JCl, JMg, JCa) was investigated in isolated perfused cortical thick ascending limb segments (cTAL) of the mouse nephron, using the microperfusion technique and the electron microprobe analysis to determine the ionic composition of the collected tubular fluid. Simultaneously, the transepithelial potential difference (PDte) and the transepithelial resistance (Rte) were recorded. Prior to the flux measurements cTAL segments were perfused for one hour. During this equilibration period PDte decreased significantly from +19.9 +/- 1.6 to +14.9 +/- 1.1 mV and Rte increased from 30.6 +/- 3.5 omega cm2 to 38.8 +/- 2.4 omega cm2 (n = 7), reflecting a decline in NaCl transport. After ADH was added to the bath solution at 10(-10) mol.l-1, PDte increased from +14.4 +/- 1.1 to +18.0 +/- 1.5 mV, accompanied by a rise in JNa and JCl from 205 +/- 11 to 273 +/- 19 and from 216 +/- 12 to 283 +/- 21 pmol.min-1.mm-1 (n = 7), respectively. JCa and JMg also increased from 0.81 +/- 0.07 to 1.50 +/- 0.12 and from 0.43 +/- 0.11 to 0.76 +/- 0.08 pmol.min-1.mm-1 (n = 7), respectively. All these effects were fully reversible after withdrawal of the hormone. In conclusion our data indicate that ADH stimulates divalent cation transport and NaCl transport in the cortical thick ascending limb of Henle's loop of the mouse.
利用微灌注技术和电子微探针分析来测定所收集的肾小管液的离子组成，研究了抗利尿激素（ADH）对小鼠肾单位离体灌流的皮质髓袢升支粗段（cTAL）上皮细胞跨膜Na⁺、Cl⁻、Ca²⁺和Mg²⁺净通量（JNa、JCl、JMg、JCa）的影响。同时，记录跨上皮电位差（PDte）和跨上皮电阻（Rte）。在进行通量测量之前，将cTAL段灌流1小时。在此平衡期内，PDte从+19.9±1.6显著降至+14.9±1.1 mV，Rte从30.6±3.5Ω·cm²增至38.8±2.4Ω·cm²（n = 7），这反映了NaCl转运的下降。在浴液中加入10⁻¹⁰ mol·L⁻¹的ADH后，PDte从+14.4±1.1升至+18.0±1.5 mV，同时JNa和JCl分别从205±11升至273±19以及从216±12升至283±21 pmol·min⁻¹·mm⁻¹（n = 7）。JCa和JMg也分别从0.81±0.07升至1.50±0.12以及从0.43±0.11升至0.76±0.08 pmol·min⁻¹·mm⁻¹（n = 7）。撤去激素后，所有这些效应均可完全逆转。总之，我们的数据表明，ADH刺激小鼠髓袢升支粗段的二价阳离子转运和NaCl转运。
