Jia Tian-tian, Cai Zhi-min, Chen Xiao-mei, Lin Zhi-jie, Huang Xiao-li, Chen Xi, Chen Guo-nan
Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Biosens Bioelectron. 2009 Sep 15;25(1):263-7. doi: 10.1016/j.bios.2009.06.030. Epub 2009 Jun 26.
An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)(3)(2+)-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10(-7) to 10(-2) M), low detection limit (5.0x10(-8) M) and good stability.
本研究对一种基于钌（联吡啶）（3）（2 +）掺杂二氧化硅纳米颗粒（RuSiNPs）的电化学发光乙醇生物传感器进行了研究。该生物传感器是一种修饰的玻碳电极，其中乙醇脱氢酶与RuSiNPs交联，然后使用壳聚糖固定在电极表面。结果表明，该生物传感器在乙醇测定过程中表现出优异的性能，线性范围宽（10（-7）至10（-2）M），检测限低（5.0×10（-8）M）且稳定性良好。
