将乙醇脱氢酶和三（2,2'-联吡啶）钌（II）固定在溶胶-凝胶杂化材料中的电化学发光生物传感器。

Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material.

作者信息

Xu Zhiai, Guo Zhihui, Dong Shaojun

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

出版信息

Biosens Bioelectron. 2005 Sep 15;21(3):455-61. doi: 10.1016/j.bios.2004.10.032. Epub 2004 Dec 18.

DOI:10.1016/j.bios.2004.10.032

PMID:16076435

Abstract

An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2'-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2'-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5x10(-5) to 5.0x10(-2) M and detection limit was 1.0x10(-5) M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.

摘要

基于电化学发光检测的乙醇生物传感器被研制出来。电化学发光试剂三（2,2'-联吡啶）钌（II）和乙醇脱氢酶被固定在同一溶胶-凝胶杂化膜中。将带有4-乙烯基吡啶的共聚聚乙烯醇和阳离子交换剂Nafion引入溶胶-凝胶膜中，以提供保持酶活性和固定三（2,2'-联吡啶）钌（II）的微环境。该设计比之前的双层形式更简单。研究了扫描速率、pH值和辅因子浓度等实验条件。电化学发光强度随乙醇浓度从2.5×10⁻⁵到5.0×10⁻² M呈线性增加，检测限为1.0×10⁻⁵ M。所制备的生物传感器具有高灵敏度、宽线性范围和良好的稳定性。