Xu Zhiai, Guo Zhihui, Dong Shaojun
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Biosens Bioelectron. 2005 Sep 15;21(3):455-61. doi: 10.1016/j.bios.2004.10.032. Epub 2004 Dec 18.
An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2'-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2'-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5x10(-5) to 5.0x10(-2) M and detection limit was 1.0x10(-5) M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.
基于电化学发光检测的乙醇生物传感器被研制出来。电化学发光试剂三（2,2'-联吡啶）钌（II）和乙醇脱氢酶被固定在同一溶胶-凝胶杂化膜中。将带有4-乙烯基吡啶的共聚聚乙烯醇和阳离子交换剂Nafion引入溶胶-凝胶膜中，以提供保持酶活性和固定三（2,2'-联吡啶）钌（II）的微环境。该设计比之前的双层形式更简单。研究了扫描速率、pH值和辅因子浓度等实验条件。电化学发光强度随乙醇浓度从2.5×10⁻⁵到5.0×10⁻² M呈线性增加，检测限为1.0×10⁻⁵ M。所制备的生物传感器具有高灵敏度、宽线性范围和良好的稳定性。
