Pezzini Jerome, Cabanne Charlotte, Santarelli Xavier
EA4135, ESTBB, Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15;877(24):2443-50. doi: 10.1016/j.jchromb.2009.06.044. Epub 2009 Jul 5.
Chromatographic performances are highly influenced by operational parameters. New ion exchangers have tailored matrices providing low backpressure and allowing high flow velocity. By systematic frontal analysis and selectivity determination at different flow rates, we suggested an independent evaluation of major anion exchangers to facilitate media selection, and investigated the relationship between (i) surface modification and (ii) chromatographic performances. Structure-extended resins showed higher binding capacities compared to resins with conventional ligands directly attached to the matrix. Moreover, they maintained mainly high capacities even with extremely high flow velocities. Ligand accessibility was therefore largely enhanced, allowing proteins to interact and bind under harsh conditions. High throughput resins can be used for purification of high volume and high concentration feedstock in limited time. This results in higher productivity and could contribute to cost reduction.
色谱性能受操作参数的影响很大。新型离子交换剂具有定制的基质，可提供低背压并允许高流速。通过在不同流速下进行系统的前沿分析和选择性测定，我们建议对主要阴离子交换剂进行独立评估以促进介质选择，并研究了（i）表面改性与（ii）色谱性能之间的关系。与直接连接到基质上的具有传统配体的树脂相比，结构扩展型树脂显示出更高的结合能力。此外，即使在极高流速下，它们也主要保持高容量。因此，配体可及性大大提高，使蛋白质能够在苛刻条件下相互作用并结合。高通量树脂可用于在有限时间内纯化高体积和高浓度的原料。这导致更高的生产率，并有助于降低成本。
