Gifalli-Iughetti C, Koiffmann C P
Centro de Estudos do Genoma Humano, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Cytogenet Genome Res. 2009;125(1):46-53. doi: 10.1159/000207518. Epub 2009 Jul 14.
In order to study the intergeneric variability of the Y chromosome, we describe the hybridization of the Y chromosome of Brachytelesarachnoides, obtained by microdissection, to metaphases of Atelesbelzebuthmarginatus, Lagothrixlagothricha, and Alouatta male specimens. Brachytelesarachnoides (Atelinae) has 62 chromosomes and a very small Y chromosome. Our results showed that the Brachytelesarachnoides Y chromosome probe hybridized to Lagothrixlagothricha metaphases yielding one hybridization signal on only the tiny Y chromosome, and when hybridized with Atelesbelzebuthmarginatus metaphases it yielded one hybridization signal on two thirds of the small acrocentric Y chromosome. However, no hybridization signal was observed in Alouatta metaphases (subfamily Alouattinae), a closely related genus in the Atelidae family. Furthermore, our data support a close phylogenetic relationship among Brachyteles, Ateles, and Lagothrix and their placement in the Atelinae subfamily, but exclude Alouatta from this group indicating its placement as basal to this group.
为了研究Y染色体的属间变异性，我们描述了通过显微切割获得的蛛猴（Brachyteles arachnoides）Y染色体与绒毛蛛猴（Ateles belzebuth marginatus）、长毛吼猴（Lagothrix lagothricha）和蛛猴属（Alouatta）雄性标本中期染色体的杂交情况。蛛猴（蜘蛛猴亚科）有62条染色体和一条非常小的Y染色体。我们的结果表明，蛛猴Y染色体探针与长毛吼猴中期染色体杂交，仅在极小的Y染色体上产生一个杂交信号；与绒毛蛛猴中期染色体杂交时，在三分之二的小近端着丝粒Y染色体上产生一个杂交信号。然而，在蛛猴科近缘属绒毛猴属（Alouatta，吼猴亚科）的中期染色体上未观察到杂交信号。此外，我们的数据支持蛛猴属、绒毛蛛猴属和长毛吼猴属之间存在密切的系统发育关系，以及它们在蜘蛛猴亚科中的分类地位，但将绒毛猴属排除在该组之外，表明其处于该组的基部位置。
