Morgan Peter J, Ortinau Stefanie, Frahm Jana, Krüger Norman, Rolfs Arndt, Frech Moritz J
Albrecht-Kossel-Institute for Neuroregeneration (AKos), Centre for Mental Health Diseases, University of Rostock, Gehlsheimer Strasse, Rostock, Germany.
Neuroreport. 2009 Aug 26;20(13):1225-9. doi: 10.1097/WNR.0b013e32832fbf49.
The survival of developing dopaminergic neurons has been shown to be modulated by voltage-dependent mechanisms. Manipulation of these mechanisms in human neural progenitor cell cultures could improve the survival of immature dopaminergic neurons, and therefore aid research into pharmacological and cell replacement therapies for Parkinson's disease. Here, we examined the effect of the Na+ channel agonist veratridine on the human fetal neural progenitor ReNcell VM cell line. Neuronal differentiation was determined by immunocytochemistry, whereas patch clamp recordings showed the expression of functional voltage-gated sodium channels. Our results show that veratridine is neuroprotective in human fetal neural progenitor cells, which may benefit studies investigating neuronal development by reducing premature death amongst developing neurons.
发育中的多巴胺能神经元的存活已被证明受电压依赖性机制调控。在人类神经祖细胞培养物中操纵这些机制可提高未成熟多巴胺能神经元的存活率，从而有助于帕金森病的药理学和细胞替代疗法的研究。在此，我们研究了Na+通道激动剂藜芦定对人胎儿神经祖细胞系ReNcell VM的影响。通过免疫细胞化学确定神经元分化，而膜片钳记录显示功能性电压门控钠通道的表达。我们的结果表明，藜芦定对人胎儿神经祖细胞具有神经保护作用，这可能有助于通过减少发育中神经元的过早死亡来研究神经元发育。
