Dabbous M K, Sobhy C, Roberts A N, Brinkley B
Mol Cell Biochem. 1977 May 31;16(1):37-42. doi: 10.1007/BF01769837.
Serum-free media of minced tissue cultures of VX-2 rabbit carcinoma contained a specific collagenolytic activity capable of releasing soluble radioactive peptides from [14C]-labeled collagen fibrils. It was also capable of reducing the viscosity of acid-soluble collagen solutions by cleaving the tropocollagen (TC) molecules primarily at one site to TCA (75%) and TCB (25%) fragments. Three chromatographic fractions were separated by gel filtration: F1, (MW 85-110,000) present in larger amounts in early cultures of younger tumor tissue; F2, (MW-35-40,000) the major component with maximum production in the day 3 media of younger and advanced tumor tissues; F3, (MW 18-22,000) the minor component. Early cultures of younger tumor tissue contained a latent collagenase and were subject to trypsin activation suggesting the presence of inactive enzyme precursors or an enzyme-inhibitor complex.
VX - 2兔癌组织碎块培养的无血清培养基含有一种特定的胶原olytic活性，能够从[14C]标记的胶原纤维中释放可溶性放射性肽。它还能够通过主要在一个位点将原胶原（TC）分子切割成TCA（75%）和TCB（25%）片段来降低酸溶性胶原溶液的粘度。通过凝胶过滤分离出三个色谱级分：F1，（分子量85 - 110,000）在较年轻肿瘤组织的早期培养物中含量较多；F2，（分子量35 - 40,000）是较年轻和晚期肿瘤组织第3天培养基中产量最高的主要成分；F3，（分子量18 - 22,000）是次要成分。较年轻肿瘤组织的早期培养物含有一种潜在的胶原酶，并可被胰蛋白酶激活，这表明存在无活性的酶前体或酶 - 抑制剂复合物。
