Tsang Adrian, Butler Gregory, Powlowski Justin, Panisko Ellen A, Baker Scott E
Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West, Montréal, Québec, Canada H4B 1R6, Department of Biology, Concordia University, Montréal, Québec, Canada.
Fungal Genet Biol. 2009 Mar;46 Suppl 1:S153-S160. doi: 10.1016/j.fgb.2008.07.014.
We used computational and mass spectrometric approaches to characterize the Aspergillus niger secretome.The 11,200 gene models predicted in the genome of A. niger strain ATCC 1015 were the data source for the analysis. Depending on the computational methods used, 691 to 881 proteins were predicted to be secreted proteins. We cultured A. niger in six different media and analyzed the extracellular proteins produced using mass spectrometry. A total of 222 proteins were identified, with 39 proteins expressed under all six conditions and 74 proteins expressed under only one condition. The secreted proteins identified by mass spectrometry were used to guide the correction of about 20 gene models. Additional analysis focused on extracellular enzymes of interest for biomass processing. Of the 63 glycoside hydrolases predicted to be capable of hydrolyzing cellulose, hemicellulose or pectin, 94% of the exo-acting enzymes and only 18% of the endo-acting enzymes were experimentally detected.
我们采用计算方法和质谱方法对黑曲霉分泌组进行了表征。黑曲霉菌株ATCC 1015基因组中预测的11200个基因模型是分析的数据源。根据所使用的计算方法，预计有691至881种蛋白质为分泌蛋白。我们在六种不同培养基中培养黑曲霉，并使用质谱分析所产生的细胞外蛋白质。总共鉴定出222种蛋白质，其中39种蛋白质在所有六种条件下均表达，74种蛋白质仅在一种条件下表达。通过质谱鉴定出的分泌蛋白用于指导约20个基因模型的校正。进一步的分析集中于生物质加工中感兴趣的细胞外酶。在预计能够水解纤维素、半纤维素或果胶的63种糖苷水解酶中，实验检测到了94%的外切作用酶，而内切作用酶仅检测到18%。
