人巨细胞病毒UL138开放阅读框在临床分离株中高度保守。

Human cytomegalovirus UL138 open reading frame is highly conserved in clinical strains.

作者信息

Qi Ying, He Rong, Ma Yan-ping, Sun Zheng-rong, Ji Yao-hua, Ruan Qiang

机构信息

Virus Laboratory, Shengjing Hospital, China Medical University, Shenyang 110004, China.

出版信息

Chin Med Sci J. 2009 Jun;24(2):107-11. doi: 10.1016/s1001-9294(09)60071-7.

DOI:10.1016/s1001-9294(09)60071-7

PMID:19618608

Abstract

OBJECTIVE

To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains.

METHODS

HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains.

RESULTS

UL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities of UL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleotide mutations were substitutions. The spatial structure and post-translational modification sites of UL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV UL138 sequence variations were not definitely related with different clinical symptoms.

CONCLUSION

HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.

摘要

目的

研究人巨细胞病毒（HCMV）UL138开放阅读框（ORF）在临床分离株中的变异性。

方法

采用聚合酶链反应（PCR）扩增HCMV UL138 ORF，对PCR扩增产物直接测序，并对19株临床分离株的数据进行分析。

结果

30株临床分离株的UL138 ORF均成功扩增。与托莱多株相比，所有分离株UL138 ORF的核苷酸和氨基酸序列同一性分别为97.41%至99.41%和98.24%至99.42%。所有核苷酸突变均为替换。UL138编码蛋白的空间结构和翻译后修饰位点保守。系统发育树结果显示，HCMV UL138序列变异与不同临床症状无明确相关性。