Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Toxicon. 2010 Jan;55(1):118-25. doi: 10.1016/j.toxicon.2009.07.008. Epub 2009 Jul 18.
Cyanea nozakii Kishinouye, a jellyfish widely distributed in coastal areas of China, has garnered attention because of its stinging capacity and the resulting public health hazard. We used a recently developed technique to extract jellyfish venom from nematocysts; the present study investigates the lethality of C. nozakii venom. The nematocyst contents were extremely toxic to the grass carp, Ctenopharyngodon idellus, producing typical neurotoxin toxicity. The ID(50) was about 0.6microg protein/g fish. Toxin samples were stable when kept at -80( degrees )C, but after 48h, an 80% decline in lethality occurred at -20( degrees )C. Poor stability of the venom was observed within the range of 65-80( degrees )C and at pH 3.5. The venom was hydrolyzed by a proteolytic enzyme, trypsin. Fractionation of the venom yielded two protein bands with molecular weights of 60kDa and 50kDa. Our results provide the first evidence that C. nozakii produces lethal toxins. These characteristics highlight the need for the isolation and molecular characterization of new active toxins in C. nozakii.
刺胞动物 Cyanea nozakii Kishinouye 广泛分布在中国沿海地区，由于其蛰刺能力和由此产生的公共卫生危害而受到关注。我们使用了一种新开发的技术从刺丝囊中提取水母毒液；本研究调查了 C. nozakii 毒液的致死性。刺丝囊内容物对草鱼（Ctenopharyngodon idellus）具有极强的毒性，产生典型的神经毒素毒性。ID（50）约为 0.6μg 蛋白/g 鱼。毒素样品在-80°C 下保持稳定，但在-20°C 下，48 小时后，致死率下降 80%。在 65-80°C 和 pH 值 3.5 的范围内，毒液稳定性较差。毒液被蛋白酶胰蛋白酶水解。毒液的分级分离得到了两条分子量为 60kDa 和 50kDa 的蛋白带。我们的研究结果首次证明了 C. nozakii 产生致死毒素。这些特性突出表明需要分离和对 C. nozakii 中的新活性毒素进行分子特征分析。
