Laboratory of Cardiovascular Diseases, West China Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, People's Republic of China.
Peptides. 2009 Oct;30(10):1816-21. doi: 10.1016/j.peptides.2009.07.006. Epub 2009 Jul 18.
Peptide aptamers are molecules which can specifically bind to a given target protein and have the potential to selectively block the function of the target protein. It has been reported that a peptide aptamer (C1-1) identified from a randomized expression library specifically bound to the core protein of hepatitis B virus and inhibited viral capsid formation and DNA replication in vitro. Adenoviral systems are popular platforms for reliable gene delivery and high-level transient expression in any mammalian cell type in vitro, and have a natural tropism for the liver after systemic administration. In the present study, we explored the feasibility of gene therapy against HBV infection with adenoviral system, and found that systematic administration of recombinant adenovirus encoding the peptide aptamer (C1-1) significantly inhibited viral capsid formation, HBV DNA replication and virion production in vivo. These results suggest an efficient antiviral treatment against HBV infection by delivery of anti-HBV peptide aptamer with recombinant adenovirus.
肽适体是能够特异性结合给定靶蛋白的分子，并且具有选择性地阻断靶蛋白功能的潜力。据报道，从随机表达文库中鉴定出的一种肽适体（C1-1）特异性结合乙型肝炎病毒的核心蛋白，并在体外抑制病毒衣壳形成和 DNA 复制。腺病毒系统是一种可靠的基因传递平台，可在体外任何哺乳动物细胞类型中进行高水平瞬时表达，并且在全身给药后对肝脏具有天然的趋向性。在本研究中，我们探讨了用腺病毒系统进行抗乙型肝炎病毒感染基因治疗的可行性，发现系统给予编码肽适体（C1-1）的重组腺病毒显著抑制了体内病毒衣壳形成、HBV DNA 复制和病毒粒子产生。这些结果表明，通过用重组腺病毒递送来治疗抗乙型肝炎病毒肽适体可以有效地治疗乙型肝炎病毒感染。
