Hu Zi-You, Qi Song-Tao, Zhang Xia, Cao Qiong, Wu Bing-Yi
Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Jul;29(7):1351-3.
To construct the delta-pIRES2-EGFP plasmid and investigate its expression in HEK293 cells.
Full length cDNA of rat delta opioid receptor gene amplified from rat brain tissues using reverse transcription and nested PCR was cloned into pMD20 T vector. The delta cDNA was inserted into pIRES2-EGFP plasmid to construct the recombinant eukaryotic plasmid delta-pIRES2-EGFP, which was transfected into HEK293 cells via Lipofectamine2000. The expression of delta was examined under fluorescence microscope.
The recombinant delta-pIRES2-EGFP plasmid was successfully constructed, and high expression of delta was detected in HEK293 cells transfected by the plasmid.
delta-pIRES2-EGFP has been successfully cloned, which shows high expression of delta in HEK293 cells.
构建δ-pIRES2-EGFP质粒并研究其在HEK293细胞中的表达。
采用逆转录和巢式PCR从大鼠脑组织中扩增大鼠δ阿片受体基因的全长cDNA，克隆至pMD20 T载体。将δ cDNA插入pIRES2-EGFP质粒构建重组真核质粒δ-pIRES2-EGFP，经Lipofectamine2000转染至HEK293细胞。在荧光显微镜下检测δ的表达。
成功构建重组δ-pIRES2-EGFP质粒，在该质粒转染的HEK293细胞中检测到δ的高表达。
成功克隆了δ-pIRES2-EGFP，其在HEK293细胞中显示出δ的高表达。
