Nielsen P E, Egholm M, Berg R H, Buchardt O
Department of Biochemistry B, Panum Institute, Copenhagen, Denmark.
Science. 1991 Dec 6;254(5037):1497-500. doi: 10.1126/science.1962210.
A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.
通过在计算机模型中去除DNA的脱氧核糖磷酸骨架并用非手性聚酰胺骨架取代它，设计了一种聚酰胺核酸（PNA）。基于该模型，制备了由胸腺嘧啶连接的氨基乙基甘氨酰单元组成的寡聚物。这些寡聚物通过链置换识别双链DNA中的互补靶标。PNA-DNA杂交体的极高稳定性使得这种置换成为可能。结果表明，DNA的骨架可以被聚酰胺取代，所得寡聚物保留碱基特异性杂交。
