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聚阴离子羧乙基肽核酸（ce-PNA）：合成与DNA结合

Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding.

作者信息

Kirillova Yuliya, Boyarskaya Nataliya, Dezhenkov Andrey, Tankevich Mariya, Prokhorov Ivan, Varizhuk Anna, Eremin Sergei, Esipov Dmitry, Smirnov Igor, Pozmogova Galina

机构信息

Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia; Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.

Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.

出版信息

PLoS One. 2015 Oct 15;10(10):e0140468. doi: 10.1371/journal.pone.0140468. eCollection 2015.

DOI:10.1371/journal.pone.0140468
PMID:26469337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4607454/
Abstract

New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA.

摘要

获得了聚酰胺核酸类似物的新型聚阴离子修饰。由各自的手性α-和γ-单体合成了胸腺嘧啶十聚体，并评估了它们的对映体纯度。在此，我们展示了十聚体的合成、纯化以及通过基质辅助激光解吸电离飞行时间质谱进行的表征，还研究了十聚体的杂交特性。我们表明，修饰后的γ-S-羧乙基-T10 PNA与聚腺嘌呤DNA形成了稳定的三链体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/3a52d49a4131/pone.0140468.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/477241178e82/pone.0140468.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/3e9870de4d51/pone.0140468.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/65e82ec16bea/pone.0140468.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/26c06e6a9e57/pone.0140468.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/3a52d49a4131/pone.0140468.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/477241178e82/pone.0140468.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/3e9870de4d51/pone.0140468.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/65e82ec16bea/pone.0140468.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/26c06e6a9e57/pone.0140468.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630f/4607454/3a52d49a4131/pone.0140468.g005.jpg

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Influence of pendant chiral C(γ)-(alkylideneamino/guanidino) cationic side-chains of PNA backbone on hybridization with complementary DNA/RNA and cell permeability.肽核酸（PNA）主链上悬挂的手性C(γ)-(亚烷基氨基/胍基)阳离子侧链对与互补DNA/RNA杂交及细胞通透性的影响。
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Single-stranded γPNAs for in vivo site-specific genome editing via Watson-Crick recognition.
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Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications.构象受限肽核酸（PNA）的展望：对结构设计、性质及应用的见解
RSC Chem Biol. 2022 Mar 18;3(6):648-697. doi: 10.1039/d2cb00017b. eCollection 2022 Jun 8.
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Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications.发现肽核酸在生物医学应用中全部潜力的化学方法。
Beilstein J Org Chem. 2021 Jul 19;17:1641-1688. doi: 10.3762/bjoc.17.116. eCollection 2021.
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Antibacterial Peptide Nucleic Acids-Facts and Perspectives.抗菌肽核酸——事实与展望。
Molecules. 2020 Jan 28;25(3):559. doi: 10.3390/molecules25030559.
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Advanced Evanescent-Wave Optical Biosensors for the Detection of Nucleic Acids: An Analytic Perspective.用于核酸检测的先进倏逝波光学生物传感器：分析视角
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Peptide nucleic acids: Advanced tools for biomedical applications.肽核酸：生物医学应用的先进工具。
J Biotechnol. 2017 Oct 10;259:148-159. doi: 10.1016/j.jbiotec.2017.07.026. Epub 2017 Jul 29.
通过沃森-克里克识别进行体内位点特异性基因组编辑的单链γ肽核酸。
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Ex vivo gene editing of the dystrophin gene in muscle stem cells mediated by peptide nucleic acid single stranded oligodeoxynucleotides induces stable expression of dystrophin in a mouse model for Duchenne muscular dystrophy.由肽核酸单链寡脱氧核苷酸介导的肌肉干细胞中抗肌萎缩蛋白基因的体外基因编辑在杜兴氏肌营养不良小鼠模型中诱导抗肌萎缩蛋白的稳定表达。
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