聚阴离子羧乙基肽核酸（ce-PNA）：合成与DNA结合

Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding.

作者信息

Kirillova Yuliya, Boyarskaya Nataliya, Dezhenkov Andrey, Tankevich Mariya, Prokhorov Ivan, Varizhuk Anna, Eremin Sergei, Esipov Dmitry, Smirnov Igor, Pozmogova Galina

机构信息

Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia; Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.

Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.

出版信息

PLoS One. 2015 Oct 15;10(10):e0140468. doi: 10.1371/journal.pone.0140468. eCollection 2015.

New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA.