Meca G, Sospedra I, Soriano J M, Ritieni A, Valero M A, Mañes J
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of València, 46100 Burjassot, Spain.
Food Chem Toxicol. 2009 Oct;47(10):2539-43. doi: 10.1016/j.fct.2009.07.014. Epub 2009 Jul 19.
To evaluate the fusaproliferin (FUS) production, Fusariumsubglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H(2)O using gradient conditions. The purity of the fusaproliferin was verified by analytical HPLC, ultraviolet absorbance measurements, LC/MS-MS, (1)H NMR spectroscopy. The isolated FUS was shown to be free of impurities and can be used as a standard for routine analysis. The pure fusaproliferin was utilized to study the biological activity on Escherichiacoli and Staphylococcusaureus. This study demostred that FUS not showed significant antimicrobial activity against these microorganisms.
为评估镰刀菌增殖素（FUS）的产生，将亚粘团镰刀菌ITEM 2404在马铃薯液体培养基中培养，该霉菌毒素通过高效液相色谱（HPLC），使用C18半制备柱，以乙腈/H₂O为流动相并采用梯度条件进行纯化。通过分析型HPLC、紫外吸光度测量、液相色谱/质谱联用（LC/MS-MS）、¹H核磁共振光谱法验证镰刀菌增殖素的纯度。所分离的FUS显示无杂质，可作为常规分析的标准品。使用纯的镰刀菌增殖素来研究其对大肠杆菌和金黄色葡萄球菌的生物活性。该研究表明FUS对这些微生物未显示出显著的抗菌活性。
