阳离子酯卟啉在肿瘤细胞中引起高水平的光毒性，并诱导人宫颈癌细胞（HeLa细胞）凋亡。
Cationic ester porphyrins cause high levels of phototoxicity in tumor cells and induction of apoptosis in HeLa Cells.
作者信息
Wu Lin, Yang Li, Huang Jing, Zhang Lixia, Weng Xiaocheng, Zhang Xiaolian, Shen Chao, Zhou Xiang, Zheng Congyi
机构信息
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, P. R. China.
出版信息
Chem Biodivers. 2009 Jul;6(7):1066-76. doi: 10.1002/cbdv.200800173.
A series of cationic ester porphyrins are much more cytotoxic to tumor cells than photofrin, meso-tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP4), and cisplatin. The lowest IC(50) value for SGC7901 is ca. 6 nM in vitro with irradiation. These porphyrins also exhibited the most potent photo-induced cytotoxicity without photobleaching. HeLa Cell apoptosis induced by cationic ester porphyrins after illumination was examined by flow cytometric analysis, staining assays with propidium iodide and annexin V FITC-PI, and further confirmed by observing morphological changes in the cells. The result of this study indicates that these cationic ester porphyrins may be applied in photodynamic therapy (PDT) in the future.
一系列阳离子酯卟啉对肿瘤细胞的细胞毒性比光敏素、中-四(1-甲基吡啶鎓-4-基)卟啉(TMPyP4)和顺铂要强得多。SGC7901的最低半数抑制浓度(IC50)值在体外光照条件下约为6 nM。这些卟啉还表现出最强的光诱导细胞毒性且无光漂白现象。通过流式细胞术分析、碘化丙啶和膜联蛋白V FITC-PI染色试验检测了光照后阳离子酯卟啉诱导的HeLa细胞凋亡，并通过观察细胞形态变化进一步证实。本研究结果表明，这些阳离子酯卟啉未来可能应用于光动力疗法(PDT)。
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