Grin'ko O M, Zverev V V, Kaloshin A A, Mikhaĭlova N A, Arzumanian V G
Zh Mikrobiol Epidemiol Immunobiol. 2009 May-Jun(3):85-9.
To study physiologic, biochemical as well as antagonistic characteristics of isolated strain of Bacillus pumilus in comparison with known spore-forming bacteria from B. subtilis species.
Four strains of Bacillus spp. were used in the study: B. subtilis 3H, B. subtilis 534, B. subtilis 1719, B. pumilus isolated from the environment. The following strains from the collection of Tarasevich State Institute of Standardization and Control for Immunobiologicals were used for determining of antagonistic activity: Staphylococcus xylosus 25, Proteus mirabilis 24a, S. aureus "Nikiforov", S. aureus "Filippov", P. vulgaris 177, Shigella flexneri 337, Escherichia coil O111:H55, S. sonnei 170, Pseudomonas aeruginosa 9022, Candida albicans 690; clinical test-strains of yeast fungi: C. albicans (3 strains), C. haemuloni, C. tropicalis, Rhodotorula spp., Debaryomyces hansenii.
Identification of isolated strain on combination of morphologic, tinctorial, cultural and biochemical characteristics showed that studied strain belonged to B. pumilus species. It did not have hemolytic and lecitinase activities, was resistant to several groups of antibiotics and had index of adhesion 1.76 +/- 2.5. In experiments in vivo the strain was non-toxic, non-toxigenic and avirulent. In preparations of isolated DNA, plasmids were not found. B. pumilus had high antagonistic effect against opportunistic and pathogenic bacteria as well as yeast fungi. Comparative assessment of tested strains from Bacillus genus showed that the isolated strain was not inferior than other representatives of spore-forming bacteria on spectrum and intensity of aforementioned characteristics.
Low-adhesive, safe, plasmid-less strain of B. pumilus, which have intensive antagonistic properties, could be considered as a candidate for the development of new probiotic drugs for medical or veterinary use.
研究短小芽孢杆菌分离株的生理、生化及拮抗特性，并与枯草芽孢杆菌属已知产芽孢细菌进行比较。
本研究使用了四株芽孢杆菌属菌株：枯草芽孢杆菌3H、枯草芽孢杆菌534、枯草芽孢杆菌1719、从环境中分离的短小芽孢杆菌。以下来自塔拉谢维奇国家免疫生物学标准化与控制研究所保藏的菌株用于拮抗活性测定：木糖葡萄球菌25、奇异变形杆菌24a、金黄色葡萄球菌“尼基福罗夫”、金黄色葡萄球菌“菲利波夫”、普通变形杆菌177、福氏志贺菌337、大肠埃希菌O111:H55、宋内志贺菌170、铜绿假单胞菌9022、白色念珠菌690；酵母真菌临床测试菌株：白色念珠菌（3株）、哈氏血色酵母菌、热带念珠菌、红酵母属、汉逊德巴利酵母。
通过形态、染色、培养和生化特性相结合对分离株进行鉴定，结果表明所研究菌株属于短小芽孢杆菌种。它没有溶血和卵磷脂酶活性，对几类抗生素具有抗性，黏附指数为1.76±2.5。在体内实验中，该菌株无毒、不产毒素且无致病性。在分离的DNA制剂中未发现质粒。短小芽孢杆菌对机会致病菌和病原菌以及酵母真菌具有高度拮抗作用。对芽孢杆菌属测试菌株的比较评估表明，分离株在上述特性的谱和强度方面不低于产芽孢细菌的其他代表菌株。
低黏附性、安全、无质粒的短小芽孢杆菌菌株具有强烈的拮抗特性，可被视为开发用于医学或兽医学的新型益生菌药物的候选菌株。
