Madueño L, Coppotelli B M, Morelli I S
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Calle 50 y 115, La Plata, Pcia. de Buenos Aires (1900), Argentina.
Rev Argent Microbiol. 2009 Apr-Jun;41(2):65-72.
The effect of the inoculant strain Sphingomonas paucimobilis 20006FA on the bacterial composition of a phenanthrene-degrading consortium obtained from a pristine soil in sequencing batch cultures was studied. Inoculated (F200+1) and non-inoculated (F200) phenanthrene-degrading consortia, were obtained. Bacterial diversity of consortia was studied at cultivable (phenotype and genotype characterization) and non-cultivable (PCR-DGGE) levels. During the successive cultures, a loss in the phenanthrene-degrading capacity and a decrease in the bacterial diversity were observed in both consortia. Although inoculation did not produce any significant changes in the consortia phenanthrene-degrading capacity (29.9% F200 and 27.6% F200+1), it did produce changes in the bacterial composition, showing a differential structural dynamics in the DGGE profiles of the inoculated consortium. In both consortia, a dominant band placed at the same position as that of the DNA of the inoculant strain in the DGGE gel could be observed. However, isolated cultures from the consortia which had an identical band position to that of S. paucimobilis 20006FA in the PCR-DGGE profile showed low similarity with respect to the inoculant strain (RAPD).
研究了接种菌株少动鞘氨醇单胞菌20006FA对从原始土壤中获得的菲降解菌群在序批式培养中的细菌组成的影响。获得了接种（F200 + 1）和未接种（F200）的菲降解菌群。在可培养（表型和基因型表征）和不可培养（PCR - DGGE）水平上研究了菌群的细菌多样性。在连续培养过程中，两个菌群中均观察到菲降解能力的丧失和细菌多样性的降低。尽管接种并未使菌群的菲降解能力产生任何显著变化（F200为29.9%，F200 + 1为27.6%），但确实使细菌组成发生了变化，在接种菌群的DGGE图谱中显示出不同的结构动态。在两个菌群中，均可观察到一条位于DGGE凝胶中与接种菌株DNA相同位置的优势条带。然而，从菌群中分离出的在PCR - DGGE图谱中与少动鞘氨醇单胞菌20006FA具有相同条带位置的培养物与接种菌株的相似性较低（RAPD）。
