[白色念珠菌中铁还原酶FRP1基因的功能]
[Function of ferric reductase FRP1 gene in Candida albicans].
作者信息
Liang Yong, Zheng Wen, Wei Dongsheng, Xing Laijun, Li Mingchun
机构信息
Key Laboratory of Molecular and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Mar;49(3):337-42.
UNLABELLED
The ability of iron acquisition in Candida albicans has an effect on its growth and pathogenesis. Ferric reductases are important components of high affinity iron acquisition system in C. albicans.
OBJECTIVE
The aim of this study was to elucidate the function of FRP1 (Ferric reductase protein).
METHODS
FRP1 gene expression was detected under low-iron and high-iron conditions by Northern blot. We used the method of PCR-directed gene disruption to construct frp 1 null mutant and then the phenotypes of frp1delta/delta mutant were characterized.
RESULTS
Low-iron condition induced FRP1 gene expression. frp1delta/delta mutant showed no growth on low-iron media compared to wild-type strains on solid plates.
CONCLUSION
FRP1 protein is probably the main ferric reductase under low-iron condition.
未标记
白色念珠菌获取铁的能力对其生长和致病机制有影响。铁还原酶是白色念珠菌高亲和力铁获取系统的重要组成部分。
目的
本研究旨在阐明FRP1（铁还原酶蛋白）的功能。
方法
通过Northern印迹法检测低铁和高铁条件下FRP1基因的表达。我们采用PCR定向基因敲除方法构建frp1缺失突变体，然后对frp1delta/delta突变体的表型进行表征。
结果
低铁条件诱导FRP1基因表达。与固体平板上的野生型菌株相比，frp1delta/delta突变体在低铁培养基上不生长。
结论
FRP1蛋白可能是低铁条件下的主要铁还原酶。
Zotero 插件