Guo Guijie, Sun Shuhong, Cui Zhizhong
College of Animal Science and Technology,Shandong Agricultural University, Tai'an 271018, China.
Wei Sheng Wu Xue Bao. 2009 Mar;49(3):400-4.
To study the source and trend in evolution of ALV-J strains from layers in China.
The genomic DNA extracted from chicken embryo fibroblasts (CEF) infected by ALV-J strain SD07LK1 isolated from layers was used as template to amplify the ALV-J proviral DNA by PCR. Nine continuous and overlapping fragments were amplified with nine pairs of primers according to published sequences, then cloned into the T vector and sequenced.
The complete sequence of the whole genome of ALV-J Chinese strain SD07LK1 was first established.
Comparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.
研究中国蛋鸡中禽白血病病毒J亚群（ALV-J）毒株的来源及进化趋势。
以从蛋鸡中分离得到的ALV-J毒株SD07LK1感染鸡胚成纤维细胞（CEF）后提取的基因组DNA为模板，通过PCR扩增ALV-J前病毒DNA。根据已发表序列设计9对引物，扩增9个连续且重叠的片段，然后克隆至T载体并测序。
首次测定了中国ALV-J毒株SD07LK1的全基因组序列。
利用DNAstar软件将SD07LK1序列与其他禽白血病病毒毒株序列进行比较，结果显示ALV-J的gag和pol基因相对保守，所有毒株的核苷酸同源性均超过95.0%。然而，env基因的同源性仅在88.6%至94.0%之间。
