[DNA依赖性蛋白激酶催化亚基表达与非小细胞肺癌细胞系放射敏感性的相关性]

[Correlation of DNA-dependent protein kinase catalytic subunit expression to radiosensitivity of non-small cell lung cancer cell lines].

作者信息

Pan Yi, Li Wei-Xiong, Li Jun-Ming, Zhu Jian-Quan, Liang Yi-Qiang, Guo Ai-Lin

机构信息

Department of Radiation Oncology, Guangdong Provincial People's Hospital, Guangdong, 510080, People's Republic of China.

出版信息

Ai Zheng. 2009 Jul;28(7):714-7. doi: 10.5732/cjc.009.10013.

DOI:10.5732/cjc.009.10013

PMID:19624897

Abstract

BACKGROUND AND OBJECTIVE

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) plays an important role in repairing irradiation-induced DNA double-strand break (DSB), and affects the radiosensitivity of tissue cells. This study was to detect the expression of DNA-PKcs in different non-small cell lung cancer (NSCLC) cell lines and evaluate its correlation to radiosensitivity.

METHODS

The content and activity of DNA-PKcs in five NSCLC cell lines A549, H1299, L78, PGCL3 and H460 were measured by Western blot and the DNA-PK activity assay. Cell survival was analyzed using clonogenic formation assay.

RESULTS

The radiosensitivities of five NSCLC cell lines were different. The values of survival fraction at 2 Gy (SF2) were 0.74 in A549 cells, 0.25 in H1299 cells, 0.21 in H460 cells, 0.48 in PGCL3 cells, and 0.58 in L78 cells. The protein levels of DNA-PKcs were 3.26+/-0.98 in A549 cells, 0.51+/-0.07 in L78 cells, 0.51+/-0.11 in H1299 cells, 0.86+/-0.23 in H460 cells, and 2.60+/-0.76 in PGCL3 cells. The activity values of DNA-PKcs were 8.30+/-1.03 in A549 cells, 2.45+/-0.52 in H1299 cells, 0.11+/-0.02 in H460 cells, 4.13+/-0.87 in PGCL3 cells, and 0.42+/-0.07 in L78 cells. In adenocarcinoma and large cell carcinoma cell lines, SF2 were correlated to DNA-PKcs content (P<0.05, r=0.95) and activity (P=0.03, r=0.98).

CONCLUSION

DNA-PKcs is an important factor to predict the radiosensitivity in adenocarcinoma and large cell lung cancer cell lines.

摘要

背景与目的

DNA依赖蛋白激酶催化亚基（DNA-PKcs）在修复辐射诱导的DNA双链断裂（DSB）中起重要作用，并影响组织细胞的放射敏感性。本研究旨在检测DNA-PKcs在不同非小细胞肺癌（NSCLC）细胞系中的表达，并评估其与放射敏感性的相关性。

方法

采用蛋白质免疫印迹法和DNA-PK活性检测法测定5种NSCLC细胞系A549、H1299、L78、PGCL3和H460中DNA-PKcs的含量和活性。采用克隆形成试验分析细胞存活率。

结果

5种NSCLC细胞系的放射敏感性不同。2 Gy时的存活分数（SF）值在A549细胞中为0.74，在H1299细胞中为0.25，在H460细胞中为0.21，在PGCL3细胞中为0.48，在L78细胞中为0.58。DNA-PKcs的蛋白水平在A549细胞中为3.26±0.98，在L78细胞中为0.51±0.07，在H1299细胞中为0.51±0.11，在H460细胞中为0.86±0.23，在PGCL3细胞中为2.60±0.76。DNA-PKcs的活性值在A549细胞中为8.30±1.03，在H1299细胞中为2.45±0.52，在H460细胞中为0.11±0.02，在PGCL3细胞中为4.13±0.87，在L（此处原文有误，应该是L78）细胞中为0.42±0.07。在腺癌和大细胞癌细胞系中，SF与DNA-PKcs含量（P<0.05，r=0.95）和活性（P=0.03，r=0.98）相关。