Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
J Pharm Biomed Anal. 2010 Jul 8;52(3):372-6. doi: 10.1016/j.jpba.2009.06.028. Epub 2009 Jun 21.
A method based on a capillary electrophoresis with laser induced fluorescence detection was developed and validated for simultaneous separation of doxorubicin (DOX) and liposomal encapsulated DOX. The separation was accomplished using a fused silica capillary (60cm in total length, 75microm I.D.) and potassium phosphate buffer [12.5mM, pH 7.4] as the running buffer. The effect of sample preparation conditions on maintaining liposomal integrity was also investigated. The limit of detection for DOX was 0.1microg/ml and the precision and accuracy of CE/LIF method was within the ranges of FDA guidelines. The validated method was successfully used to quantify DOX in human plasma using a direct injection of a 4-fold dilution of spiked liposomal DOX in human plasma.
建立并验证了一种基于毛细管电泳激光诱导荧光检测的方法，用于同时分离阿霉素（DOX）和脂质体包裹的 DOX。采用熔融石英毛细管（总长度 60cm，内径 75μm）和磷酸钾缓冲液[12.5mM，pH7.4]作为运行缓冲液进行分离。还研究了样品制备条件对保持脂质体完整性的影响。DOX 的检测限为 0.1μg/ml，CE/LIF 方法的精密度和准确度在 FDA 指南规定的范围内。该经过验证的方法成功地用于通过直接注射 4 倍稀释的人血浆中加入的脂质体 DOX 来定量人血浆中的 DOX。
