采用毛细管电泳和激光诱导荧光法同时分析脂质体阿霉素和阿霉素。
Simultaneous analysis of liposomal doxorubicin and doxorubicin using capillary electrophoresis and laser induced fluorescence.
机构信息
Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
出版信息
J Pharm Biomed Anal. 2010 Jul 8;52(3):372-6. doi: 10.1016/j.jpba.2009.06.028. Epub 2009 Jun 21.
Abstract
A method based on a capillary electrophoresis with laser induced fluorescence detection was developed and validated for simultaneous separation of doxorubicin (DOX) and liposomal encapsulated DOX. The separation was accomplished using a fused silica capillary (60cm in total length, 75microm I.D.) and potassium phosphate buffer [12.5mM, pH 7.4] as the running buffer. The effect of sample preparation conditions on maintaining liposomal integrity was also investigated. The limit of detection for DOX was 0.1microg/ml and the precision and accuracy of CE/LIF method was within the ranges of FDA guidelines. The validated method was successfully used to quantify DOX in human plasma using a direct injection of a 4-fold dilution of spiked liposomal DOX in human plasma.
摘要
建立并验证了一种基于毛细管电泳激光诱导荧光检测的方法，用于同时分离阿霉素（DOX）和脂质体包裹的 DOX。采用熔融石英毛细管（总长度 60cm，内径 75μm）和磷酸钾缓冲液[12.5mM，pH7.4]作为运行缓冲液进行分离。还研究了样品制备条件对保持脂质体完整性的影响。DOX 的检测限为 0.1μg/ml，CE/LIF 方法的精密度和准确度在 FDA 指南规定的范围内。该经过验证的方法成功地用于通过直接注射 4 倍稀释的人血浆中加入的脂质体 DOX 来定量人血浆中的 DOX。
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