Pronina Veronica V, Kostryukova Lyubov V, Ivanov Sergey V, Tichonova Elena G, Archakov Alexander I, Shumyantseva Victoria V
Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
Faculty of Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
Biomedicines. 2025 Apr 20;13(4):996. doi: 10.3390/biomedicines13040996.
Our study brings a new method to properly evaluating drug efficacy at the non-invasive in vitro level. In this work, the electrochemical mediator-free and reagent-free analysis of cell lines based on the registration of electrochemical profiles of membrane proteins was developed. We studied the specificity of cell lines Wi-38 and HepG2 and the toxic effects of drugs on cell-on-electrode systems. A linear dependence of the peak current on the concentration of cells applied to the electrode in the range from 1 × 10 to 6 × 10 cells/electrode was registered (R 0.932 for Wi-38 and R 0.912 for HepG2). The water-soluble form of phosphatidylcholine (wPC) nanoparticles recommended for atherosclerosis treatment and prevention of cardiovascular diseases did not show a toxic effect on the human fibroblast cells, Wi-38, or the human hepatocellular carcinoma cells, HepG2, at sufficiently high concentrations (such as 0.1-1 mg/mL). The antitumor drug doxorubicin, at concentrations of 3 and 10 μg/mL, showed a pronounced toxic effect on the tested cell lines, where the percentage of living cells was 50-55%. A comparative analysis of the cytotoxicity of wPC (0.1-1 mg/mL) and doxorubicin (3-10 μg/mL) on the cell lines Wi-38 and HepG2 using the MTT test and electrochemical approach for the registration of cells showed their clear adequacy.
我们的研究带来了一种在非侵入性体外水平正确评估药物疗效的新方法。在这项工作中，基于膜蛋白电化学图谱的记录，开发了无电化学介质和无试剂的细胞系分析方法。我们研究了Wi-38和HepG2细胞系的特异性以及药物对电极上细胞系统的毒性作用。记录了在1×10至6×10个细胞/电极范围内施加到电极上的细胞浓度与峰值电流之间的线性关系（Wi-38的R为0.932，HepG2的R为0.912）。推荐用于动脉粥样硬化治疗和预防心血管疾病的水溶性磷脂酰胆碱（wPC）纳米颗粒在足够高的浓度（如0.1-1mg/mL）下，对人成纤维细胞Wi-38或人肝癌细胞HepG2未显示出毒性作用。抗肿瘤药物阿霉素在3和10μg/mL的浓度下，对测试的细胞系显示出明显的毒性作用，其中活细胞百分比为50-55%。使用MTT试验和电化学方法对细胞进行记录，对wPC（0.1-1mg/mL）和阿霉素（3-10μg/mL）在Wi-38和HepG2细胞系上的细胞毒性进行比较分析，结果显示它们明显具有可比性。
