Eipper B A, Mains R E
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Am J Clin Nutr. 1991 Dec;54(6 Suppl):1153S-1156S. doi: 10.1093/ajcn/54.6.1153s.
The biosynthesis of all alpha-amidated peptides requires the participation of peptidylglycine alpha-amidating monooxygenase (PAM), a bifunctional enzyme dependent on molecular oxygen, ascorbate, and copper. In the AtT-20 corticotrope cell line, reduced concentrations of PAM and ascorbate can make alpha-amidation the rate-limiting step in the production of bioactive product peptide. Production of various peptides thought to play autocrine and paracrine roles may be affected by ascorbate availability.
所有α-酰胺化肽的生物合成都需要肽基甘氨酸α-酰胺化单加氧酶（PAM）的参与，PAM是一种依赖分子氧、抗坏血酸和铜的双功能酶。在AtT-20促肾上腺皮质激素细胞系中，PAM和抗坏血酸浓度降低会使α-酰胺化成为生物活性产物肽产生过程中的限速步骤。各种被认为起自分泌和旁分泌作用的肽的产生可能会受到抗坏血酸可用性的影响。
