Department of Life Science, Sookmyung Women's University, Yongsan-gu, Seoul, 140-742, Korea.
Aesthetic Plast Surg. 2009 Sep;33(5):722-9. doi: 10.1007/s00266-009-9394-0. Epub 2009 Jul 21.
Recently, many plastic surgeons have been using adipogenic-differentiated cell implantation for remodeling scars in patients. However, this technique is not a long-term solution because implanted cells disappear gradually. Therefore, we investigated a method to increase the grafted cell preservation rate by using an effective adjuvant, botulinum toxin.
The adipogenic-differentiated cells were subcutaneously injected in the dorsal area of C57/BL6 mice with or without botulinum toxin. Two and six weeks later we analyzed the residual volume and confirmed the characteristics of the implanted cells by real-time RT-PCR and immunohistochemistry.
Two and six weeks after transplantation we found that the residual volume of the transplantation site was higher in the botulinum toxin-treated group than in the untreated group. We also confirmed that the residual transplanted area has characteristics of adipogenic tissue by histological analysis. Next, to determine the mechanism related to the enhanced preservation rate of grafted cells via treatment with botulinum toxin, we performed immunohistochemical staining for the angiogenesis-related marker CD31. We found that CD31 expression was higher in the botulinum toxin-treated group than in the untreated group.
We have shown that in vivo grafted adipocyte cell preservation can be enhanced by treatment with botulinum toxin as an adjuvant. We suggest that botulinum toxin further increases this graft preservation rate by enhancing angiogenesis.
最近，许多整形外科医生一直在使用脂肪细胞分化细胞植入术来重塑患者的疤痕。然而，这种技术不是长期解决方案，因为植入的细胞会逐渐消失。因此，我们研究了一种通过使用有效的佐剂肉毒毒素来提高移植细胞保存率的方法。
将脂肪细胞分化细胞皮下注射到 C57/BL6 小鼠的背部区域，同时给予或不给予肉毒毒素。2 周和 6 周后，我们通过实时 RT-PCR 和免疫组织化学分析来分析残留体积并确认植入细胞的特征。
移植后 2 周和 6 周，我们发现肉毒毒素处理组的移植部位残留体积高于未处理组。我们还通过组织学分析证实了残留的移植区域具有脂肪组织的特征。接下来，为了确定通过肉毒毒素治疗增强移植细胞保存率的相关机制，我们对与血管生成相关的标志物 CD31 进行了免疫组织化学染色。我们发现肉毒毒素处理组的 CD31 表达高于未处理组。
我们已经表明，通过使用肉毒毒素作为佐剂，体内移植的脂肪细胞保存可以得到增强。我们认为肉毒毒素通过增强血管生成进一步提高了这种移植物的保存率。
