p53蛋白和诱导型一氧化氮合酶在牙源性囊肿中的免疫组化表达

Immuno-histochemical expression of p53 protein and iNOS in odontogenic cysts.

作者信息

Poomsawat Sopee, Punyasingh Jirapa, Vejchapipat Paisarn

机构信息

Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

出版信息

J Med Assoc Thai. 2009 Jul;92(7):952-60.

PMID:19626816

Abstract

OBJECTIVE

The present study investigated the expression of p53 protein and iNOS in odontogenic keratocysts (OKCs), dentigerous cysts (DCs) and radicular cysts (RCs) and determined the correlation of these 2 proteins within each cyst type.

MATERIAL AND METHOD

p53 and iNOS in epithelial lining cells were analyzed in 20 OKCs, 20 DCs and 20 RCs using immuno-histochemistry. The percentage of positive cells was assessed and presented as mean +/-SD. The correlation between the expressions of these 2 proteins in each cyst was carried out using Spearman correlation analysis.

RESULTS

p53 was found in 95% of OKCs, 50% of DCs and 65% of RCs. p53-positive cells in OKCs (12.2 +/- 8.8%) were significantly higher than those in DCs (1.4 +/- 3.2%) and RCs (1.3 +/- 2.4%). iNOS was found in all examined lesions. Statistically, iNOS-positive cells in RCs (86.9 +/- 9.9%) were lower than those in OKCs (94.8 +/- 3.5%) and DCs (96.1 +/- 4.4%). No correlation between the expression of p53 and iNOS was found in each cyst.

CONCLUSION

p53 was over expressed in OKCs compared with other lesions. RCs showed the lowest expression of iNOS.

摘要

目的

本研究调查了p53蛋白和诱导型一氧化氮合酶（iNOS）在牙源性角化囊肿（OKC）、含牙囊肿（DC）和根尖囊肿（RC）中的表达情况，并确定了这两种蛋白在每种囊肿类型中的相关性。

材料与方法

采用免疫组织化学方法分析了20例OKC、20例DC和20例RC上皮衬里细胞中的p53和iNOS。评估阳性细胞百分比，并以平均值±标准差表示。使用Spearman相关性分析对每种囊肿中这两种蛋白的表达之间的相关性进行分析。

结果

在95%的OKC、50%的DC和65%的RC中发现了p53。OKC中p53阳性细胞（12.2±8.8%）显著高于DC（1.4±3.2%）和RC（1.3±2.4%）。在所有检查的病变中均发现了iNOS。统计学上，RC中iNOS阳性细胞（86.9±9.9%）低于OKC（94.8±3.5%）和DC（96.1±4.4%）。在每种囊肿中未发现p53和iNOS表达之间的相关性。